Effects of Peroxisome Proliferator-Activated Receptor γ on Modulating Angiopoietin-Like Protein 4 Synthesis in Caco-2 Cells Exposed to Clostridium butyricum
- 作者: Zhao X.1, Huang H.2, Shi S.3
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隶属关系:
- College of Agriculture and Forestry Science, Linyi University
- Shandong Longda Biotechnology Co., Ltd.
- Poultry Institute, Chinese Academy of Agricultural Sciences
- 期: 卷 57, 编号 3 (2023)
- 页面: 501-502
- 栏目: МОЛЕКУЛЯРНАЯ БИОЛОГИЯ КЛЕТКИ
- URL: https://journals.rcsi.science/0026-8984/article/view/138630
- DOI: https://doi.org/10.31857/S0026898423030217
- EDN: https://elibrary.ru/CIBNGM
- ID: 138630
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详细
Angiopoietin-like protein 4 (ANGPTL4) is considered to be one of the important circulating mediators linking intestinal microorganisms and host lipid metabolism. The objective of this study was to assess the effects of peroxisome proliferator-activated receptor γ (PPARγ) on modulating ANGPTL4 synthesis in Caco-2 cells exposed to Clostridium butyricum. The viability of Caco-2 cells and the expression of PPARγ and ANGPTL4 in Caco-2 cells were detected after the Caco-2 cells were co-cultured with C. butyricum at the concentration of 1 × 106, 1 × 107 and 1 × 108 CFU/mL. The results showed that cell viability was enhanced by C. butyricum. Besides, PPARγ and ANGPTL4 expression and secretion in Caco-2 cells was significantly increased by 1 × 107 and 1 × 108 CFU/mL of C. butyricum. Furthermore, the effects of PPARγ on modulating ANGPTL4 synthesis in Caco-2 cells regulated by 1 × 108 CFU/mL of C. butyricum was also be expounded in PPARγ activation/inhibition model based on Caco-2 cells and via ChIP technique. It was found that C. butyricum promoted the binding of PPARγ to the PPAR binding site (chr19: 8362157-8362357, located upstream of the transcriptional start site of angptl4) of the angptl4 gene in Caco-2 cells. However, the PPARγ was not the only way for C. butyricum to stimulate ANGPTL4 production. Taken together, PPARγ played a role in the regulation of ANGPTL4 synthesis by C. butyricum in Caco-2 cells.
作者简介
X. Zhao
College of Agriculture and Forestry Science, Linyi University
编辑信件的主要联系方式.
Email: kity850814@163.com
China, 276000, Linyi, Shandong
H. Huang
Shandong Longda Biotechnology Co., Ltd.
Email: ssr236@163.com
China, 276400, Linyi, Shandong
S. Shi
Poultry Institute, Chinese Academy of Agricultural Sciences
编辑信件的主要联系方式.
Email: ssr236@163.com
China, 271018 , Yangzhou, Jiangsu