Genotype-Specific Features of Cold-Induced Sweetening Process Regulation in Potato Varieties Nikulinsky, Symphony, Nevski

A. A. Egorova; Егорова А. А.; I. A. Saboiev; Сабоиев И. А.; N. E. Kostina; Костина Н. Е.; D. D. Kuvaeva; Куваева Д. Д.; A. B. Shcherban; Щербань А. Б.; S. M. Ibragimova; Ибрагимова С. М.; E. A. Salina; Салина Е. А.; A. V. Kochetov; Кочетов А. В.

doi:10.31857/S0026898423020064

Genotype-Specific Features of Cold-Induced Sweetening Process Regulation in Potato Varieties Nikulinsky, Symphony, Nevski

Authors: Egorova A.A.¹^,2, Saboiev I.A.², Kostina N.E.¹, Kuvaeva D.D.¹, Shcherban A.B.¹^,2, Ibragimova S.M.¹^,2, Salina E.A.², Kochetov A.V.¹
Affiliations:
1. Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences
2. Kurchatov Genomic Center of the Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences
Issue: Vol 57, No 2 (2023)
Pages: 197-208
Section: БИОНФОРМАТИКА, БИОНЖЕНЕРИЯ И БИОТЕХНОЛОГИЯ
URL: https://journals.rcsi.science/0026-8984/article/view/138623
DOI: https://doi.org/10.31857/S0026898423020064
EDN: https://elibrary.ru/EFXYKD
ID: 138623

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Abstract
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Abstract

Increasing the shelf life of potato tubers demands their storage under low temperature. However, storage at low temperatures causes activation of cold-protective mechanism, so-called cold-induced sweetening (CIS). During this process, the starch is converted into reducing sugars such as glucose and fructose. Upon processing at high-temperature, these sugars react with free amino acids, resulting in brown, bitter-tasting products, which reduces the consumer value of potatoes. In this study, we performed expression analysis of genes associated with cold-induced sweetening in potato tubers: vacuolar invertase (Pain-1), sucrose-synthase (SUS4), invertase inhibitor (InvInh2). Potato varieties Nikulinsky, Symphony, Nevski were used. All three varieties were found to accumulate sugars at low temperatures, the maximum accumulation of reducing sugars being observed at 4°C. It was found that the expression pattern of genes associated with cold-induced sweetening differs depending on the variety and storage duration. The increased expression of vacuolar invertase and its inhibitor is more pronounced at the beginning of storage period while the increased expression of sucrose synthase is more pronounced after three months of storage. At early storage periods, high expression of invertase and low expression of inhibitor is observed in Dutch variety Symphony, and vice versa in Russian varieties Nikulinsky and Nevsky. The participation of the studied genes in the process of cold-induced sweetening is discussed.

Keywords

Solanum tuberosum L., potato, cold-induced sweetening, regulation, expression, vacuolar invertase, sucrose synthase

References

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