Differential Expression of a Foreign Gene in Arabidopsis Mitochondria  in organello

V. I. Tarasenko; Тарасенко В. И.; T. A. Tarasenko; Тарасенко Т. А.; I. V. Gorbenko; Горбенко И. В.; Yu. M. Konstantinov; Константинов Ю. М.; M. V. Koulintchenko; Кулинченко М. В.

doi:10.31857/S002689842303014X

Differential Expression of a Foreign Gene in Arabidopsis Mitochondria in organello

Авторлар: Tarasenko V.¹, Tarasenko T.¹, Gorbenko I.¹, Konstantinov Y.¹, Koulintchenko M.¹^,2
Мекемелер:
1. Siberian Institute of Plant Physiology and Biochemistry Siberian Branch, Russian Academy of Sciences
2. Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Center, Russian Academy of Sciences
Шығарылым: Том 57, № 3 (2023)
Беттер: 460-470
Бөлім: ГЕНОМИКА. ТРАНСКРИПТОМИКА
URL: https://journals.rcsi.science/0026-8984/article/view/138614
DOI: https://doi.org/10.31857/S002689842303014X
EDN: https://elibrary.ru/CHUECL
ID: 138614

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Рұқсат жабық

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Аннотация
Авторлар туралы
Әдебиет тізімі
Қосымша файлдар
Статистика

Аннотация

Genetic transformation of higher eukaryotes mitochondria in vivo is one of the unresolved and important problems. For efficient expression of foreign genetic material in mitochondria, it is necessary to select regulatory elements that ensure a high level of transcription and transcript stability. This work is aimed at studying the effectiveness of regulatory elements of mitochondrial genes flanking exogenous DNA using the phenomenon of natural competence of plant mitochondria. For this purpose, genetic constructs carrying the GFP gene under the control of the promoter regions of the RRN26 or COX1 genes and one of the two 3'-untranslated regions (3'-UTR) of mitochondrial genes were imported into isolated Arabidopsis mitochondria, followed by transcription in organello. It was shown that the level of GFP expression under the control of promoters of the RRN26 or COX1 genes in organello correlates with the level of transcription of these genes observed in vivo. At the same time, the presence of the tRNA^Тrp sequence in the 3'-UTR leads to a higher level of the GFP transcript than the presence in this region of the 3'-UTR of the NAD4 gene containing the binding site of the MTSF1 protein. The results obtained open up prospects for creating a system for efficient transformation of the mitochondrial genome.

Негізгі сөздер

regulation of gene expression, DNA import, GFP, promoter region, untranslated region, RPOTmp, Arabidopsis thaliana mitochondria, in organello

Әдебиет тізімі

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