Enviar artigo por via de e-mail Proteomic Profile of the Bacterium Sinorhizobium meliloti Depends on Its Life Form and Host Plant Species
- Autores: Antonets K.S.1,2, Onishchuk O.P.1, Kurchak O.N.1, Volkov K.V.2, Lykholay A.N.2, Andreeva E.A.2, Andronov E.E.1,2, Pinaev A.G.1, Provorov N.A.1, Nizhnikov A.A.1,2
-
Afiliações:
- All-Russia Research Institute of Agricultural Microbiology
- St. Petersburg State University
- Edição: Volume 52, Nº 5 (2018)
- Páginas: 779-785
- Seção: Proteomics
- URL: https://journals.rcsi.science/0026-8933/article/view/163704
- DOI: https://doi.org/10.1134/S0026893318050035
- ID: 163704
Citar
Acesso aberto
Acesso está concedido
Somente assinantes
Resumo
Abstract—The importance of root nodule bacteria in biotechnology is determined by their distinctive feature: symbiotic nitrogen fixation resulting in the production of organic nitrogen-containing compounds. While interacting with host legume plants, the cells of these bacteria undergo global changes at all levels of expression of genetic information leading to the formation in root nodules of so-called bacteroids functioning as nitrogen fixation factories. The molecular mechanisms underlying plant-microbial symbiosis are actively investigated, and one of the most interesting and poorly studied aspects of this problem is the species-specificity of interaction between root nodule bacteria and host plants. In this work we have performed the proteomic analysis of the Sinorhizobium meliloti bacteroids isolated from two legume species: alfalfa (Medicago sativa L.) and yellow sweet clover (Melilotus officinalis L.). It has been shown that the S. meliloti bacteroids produce a lot of proteins (many of them associated with symbiosis) in a host-specific manner, i.e., only in certain host plant species. It has been demonstrated for the first time that the levels of expression in bacteroids of the genes encoding the ExoZ and MscL proteins responsible for the synthesis of surface lipopolysaccharides and formation of a large conductance mechanosensitive channel, respectively, depend on a host plant species that confirms the results of proteomic analysis. Overall, our data show that the regulation of bacteroid development by the host plant has species-specific features.
Palavras-chave
Sobre autores
K. Antonets
All-Russia Research Institute of Agricultural Microbiology; St. Petersburg State University
Email: ant.nizhnikov@gmail.com
Rússia, Pushkin, St. Petersburg, 196608; St. Petersburg, 199034
O. Onishchuk
All-Russia Research Institute of Agricultural Microbiology
Email: ant.nizhnikov@gmail.com
Rússia, Pushkin, St. Petersburg, 196608
O. Kurchak
All-Russia Research Institute of Agricultural Microbiology
Email: ant.nizhnikov@gmail.com
Rússia, Pushkin, St. Petersburg, 196608
K. Volkov
St. Petersburg State University
Email: ant.nizhnikov@gmail.com
Rússia, St. Petersburg, 199034
A. Lykholay
St. Petersburg State University
Email: ant.nizhnikov@gmail.com
Rússia, St. Petersburg, 199034
E. Andreeva
St. Petersburg State University
Email: ant.nizhnikov@gmail.com
Rússia, St. Petersburg, 199034
E. Andronov
All-Russia Research Institute of Agricultural Microbiology; St. Petersburg State University
Email: ant.nizhnikov@gmail.com
Rússia, Pushkin, St. Petersburg, 196608; St. Petersburg, 199034
A. Pinaev
All-Russia Research Institute of Agricultural Microbiology
Email: ant.nizhnikov@gmail.com
Rússia, Pushkin, St. Petersburg, 196608
N. Provorov
All-Russia Research Institute of Agricultural Microbiology
Email: ant.nizhnikov@gmail.com
Rússia, Pushkin, St. Petersburg, 196608
A. Nizhnikov
All-Russia Research Institute of Agricultural Microbiology; St. Petersburg State University
Autor responsável pela correspondência
Email: ant.nizhnikov@gmail.com
Rússia, Pushkin, St. Petersburg, 196608; St. Petersburg, 199034
Arquivos suplementares
