Degradation of the Biofilms of Gram-Positive and Gram-Negative Bacteria by the PAPC Serine protease from Aspergillus ochraceus

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Infections associated with biofilm formation by gram-positive and gram-negative microorganisms cause difficulty in therapy and are prone to transition into chronic forms. Approaches to degradation of the biofilm matrix are therefore in demand. In the present work, recombinant recombinant PAPC serine protease from Aspergillus ochraceus caused the degradation of mature biofilms formed by a number of gram-positive and gram-negative bacteria by 15‒20% at 50 µg/mL. At 100 µg/mL, the biomass of S. aureus and P. aeruginosa biofilms decreased by 50%. Thus, the PAPC may be a promising agent for biofilm removal and enhance the efficiency of antimicrobial therapy.

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作者简介

D. Baidamshina

Kazan (Volga Region) Federal University

编辑信件的主要联系方式.
Email: dianabaidamshina@yandex.ru
俄罗斯联邦, Kazan, 420008

A. Rafia Nasr

Kazan (Volga Region) Federal University

Email: dianabaidamshina@yandex.ru
俄罗斯联邦, Kazan, 420008

S. Komarevtsev

Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Email: dianabaidamshina@yandex.ru
俄罗斯联邦, Moscow, 117997

A. Osmolovskii

Moscow State Uniuversity

Email: dianabaidamshina@yandex.ru

Biological Faculty

俄罗斯联邦, Moscow, 1179974; Moscow, 119234

K. Miroshnikov

Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; Moscow State Uniuversity

Email: dianabaidamshina@yandex.ru

Biological Faculty

俄罗斯联邦, Moscow, 119234

A. Kayumov

Kazan (Volga Region) Federal University

Email: dianabaidamshina@yandex.ru
俄罗斯联邦, Kazan, 420008

E. Trizna

Kazan (Volga Region) Federal University

Email: dianabaidamshina@yandex.ru
俄罗斯联邦, Kazan, 420008

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2. Fig. 1. Assessment of the destruction of the total volume of biofilms and the volume of the matrix of bacterial biofilms by PAPC protease. The color is crystalline purple and Congo red.

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3. Fig. 2. The effect of PAPC (100 mcg/ml) on the integrity of the biofilm and effectiveness against S. aureus, M. luteus, E. faecalis, E. coli, K. pneumoniae and P. aeruginosa cells in the composition of the formed biofilm. On micrographs, the scale mark corresponds to 5 microns, on Z-slices — 10 microns.

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