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Degradation of the Biofilms of Gram-Positive and Gram-Negative Bacteria by the PAPC Serine protease from Aspergillus ochraceus
- Authors: Baidamshina D.R.1, Rafia Nasr A.1, Komarevtsev S.K.2, Osmolovskii A.A.3, Miroshnikov K.A.2,3, Kayumov A.R.1, Trizna E.Y.1
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Affiliations:
- Kazan (Volga Region) Federal University
- Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
- Moscow State Uniuversity
- Issue: Vol 93, No 2 (2024)
- Pages: 228-233
- Section: SHORT COMMUNICATIONS
- URL: https://journals.rcsi.science/0026-3656/article/view/262573
- DOI: https://doi.org/10.31857/S0026365624020247
- ID: 262573
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Abstract
Infections associated with biofilm formation by gram-positive and gram-negative microorganisms cause difficulty in therapy and are prone to transition into chronic forms. Approaches to degradation of the biofilm matrix are therefore in demand. In the present work, recombinant recombinant PAPC serine protease from Aspergillus ochraceus caused the degradation of mature biofilms formed by a number of gram-positive and gram-negative bacteria by 15‒20% at 50 µg/mL. At 100 µg/mL, the biomass of S. aureus and P. aeruginosa biofilms decreased by 50%. Thus, the PAPC may be a promising agent for biofilm removal and enhance the efficiency of antimicrobial therapy.
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About the authors
D. R. Baidamshina
Kazan (Volga Region) Federal University
Author for correspondence.
Email: dianabaidamshina@yandex.ru
Russian Federation, Kazan, 420008
A. Rafia Nasr
Kazan (Volga Region) Federal University
Email: dianabaidamshina@yandex.ru
Russian Federation, Kazan, 420008
S. K. Komarevtsev
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: dianabaidamshina@yandex.ru
Russian Federation, Moscow, 117997
A. A. Osmolovskii
Moscow State Uniuversity
Email: dianabaidamshina@yandex.ru
Biological Faculty
Russian Federation, Moscow, 1179974; Moscow, 119234K. A. Miroshnikov
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; Moscow State Uniuversity
Email: dianabaidamshina@yandex.ru
Biological Faculty
Russian Federation, Moscow, 119234A. R. Kayumov
Kazan (Volga Region) Federal University
Email: dianabaidamshina@yandex.ru
Russian Federation, Kazan, 420008
E. Yu. Trizna
Kazan (Volga Region) Federal University
Email: dianabaidamshina@yandex.ru
Russian Federation, Kazan, 420008
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