Degradation of the Biofilms of Gram-Positive and Gram-Negative Bacteria by the PAPC Serine protease from Aspergillus ochraceus

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Abstract

Infections associated with biofilm formation by gram-positive and gram-negative microorganisms cause difficulty in therapy and are prone to transition into chronic forms. Approaches to degradation of the biofilm matrix are therefore in demand. In the present work, recombinant recombinant PAPC serine protease from Aspergillus ochraceus caused the degradation of mature biofilms formed by a number of gram-positive and gram-negative bacteria by 15‒20% at 50 µg/mL. At 100 µg/mL, the biomass of S. aureus and P. aeruginosa biofilms decreased by 50%. Thus, the PAPC may be a promising agent for biofilm removal and enhance the efficiency of antimicrobial therapy.

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About the authors

D. R. Baidamshina

Kazan (Volga Region) Federal University

Author for correspondence.
Email: dianabaidamshina@yandex.ru
Russian Federation, Kazan, 420008

A. Rafia Nasr

Kazan (Volga Region) Federal University

Email: dianabaidamshina@yandex.ru
Russian Federation, Kazan, 420008

S. K. Komarevtsev

Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Email: dianabaidamshina@yandex.ru
Russian Federation, Moscow, 117997

A. A. Osmolovskii

Moscow State Uniuversity

Email: dianabaidamshina@yandex.ru

Biological Faculty

Russian Federation, Moscow, 1179974; Moscow, 119234

K. A. Miroshnikov

Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; Moscow State Uniuversity

Email: dianabaidamshina@yandex.ru

Biological Faculty

Russian Federation, Moscow, 119234

A. R. Kayumov

Kazan (Volga Region) Federal University

Email: dianabaidamshina@yandex.ru
Russian Federation, Kazan, 420008

E. Yu. Trizna

Kazan (Volga Region) Federal University

Email: dianabaidamshina@yandex.ru
Russian Federation, Kazan, 420008

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Supplementary files

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2. Fig. 1. Assessment of the destruction of the total volume of biofilms and the volume of the matrix of bacterial biofilms by PAPC protease. The color is crystalline purple and Congo red.

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3. Fig. 2. The effect of PAPC (100 mcg/ml) on the integrity of the biofilm and effectiveness against S. aureus, M. luteus, E. faecalis, E. coli, K. pneumoniae and P. aeruginosa cells in the composition of the formed biofilm. On micrographs, the scale mark corresponds to 5 microns, on Z-slices — 10 microns.

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