Effect of the PotN Protein on Activities of the GlnR and PotA Proteins in the Cells of Lentilactobacillus hilgardii

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Аннотация

Bacterial PII-like proteins are global regulators of nitrogen and energy metabolism, which respond to nutrient availability by binding their partner proteins, thus modulating their activity. The PotN protein from Lentilactobacillus hilgardii, a member of the new family of PII-like proteins, is capable of competitive binding of ATP and ADP, thus regulating metabolism in response to the cell energy status. Thus, under ADP excess, PotN binds this nucleotide and interacts mostly with the PotA subunit of the polyamine АВС transporter, suppressing its ATPase activity. PotN also dissociates from the transcription factor GlnR, restoring its ability to bind DNA and modulate expression of the genes of the GlnR regulon. On the contrary, in the ATP state PotN dissociates from PotA and binds to the GlnR factor.

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Авторлар туралы

Z. Iskhakova

Kazan (Volga Region) Federal University

Хат алмасуға жауапты Автор.
Email: zalinunya@mail.ru
Ресей, Kazan, 420008

D. Zhuravleva

Kazan (Volga Region) Federal University

Email: zalinunya@mail.ru
Ресей, Kazan, 420008

A. Kayumov

Kazan (Volga Region) Federal University

Email: zalinunya@mail.ru
Ресей, Kazan, 420008

Әдебиет тізімі

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2. Fig. 1. The effect of PotN on the ATPase activity of PotAc. Glutamine synthetase (GS, positive control) and bovine serum albumin (BSA, negative control) were used as controls. The graph shows the average values ± standard deviation from 3 repeats of the experiment. The significance of the differences between the PotN-PotAc and PotAc complexes was assessed by the Kruskal–Wallis method with the Shidak correction. The differences were considered significant at *p < 0.05.

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3. Fig. 2. The level of relative fluorescence of DNA. (a) — Typical MST traces of changes in the fluorescence intensity of a single DNA and a DNA-GlnR complex. (b) — A change in the intensity of DNA fluorescence in the PotN-GlnR-PotAc-DNA system in combination with various nucleotides. The significance of fluorescence differences in the presence of different nucleotides was assessed by the Student's t-test. The differences were considered significant at *p < 0.05.

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