Specific Response of Bacterial Cells to β-Ionone

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Abstract

The biological activity and mechanisms of action of the unsaturated ketone β-ionone, a volatile compound of significantinterest for biotechnology, medicine, and agriculture, were studied. Using specific lux biosensors basedon Escherichia coli MG1655, we found that β-ionone causes oxidative stress in E. coli cells by inducing expression from the PkatG and Pdps promoters, but not from the PsoxS promoter. The effects of β-ionone on the heat shock induction (expression from the PibpA and PgrpE promoters) and on DNA damage (expression from the PcolD and PdinI promoters, SOS response) in E. coli cells were significantly weaker. β-Ionone did not cause oxidative stress in the cells of the gram-positive bacterium Bacillus subtilis.

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About the authors

D. E. Sidorova

Complex of NBICS Technologies of the NRC “Kurchatov Institute”

Email: plyutaba@gmail.com
Russian Federation, Moscow, 123182

O. E. Melkina

Complex of NBICS Technologies of the NRC “Kurchatov Institute”

Email: plyutaba@gmail.com
Russian Federation, Moscow, 123182

O. A. Koksharova

Complex of NBICS Technologies of the NRC “Kurchatov Institute”; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University

Email: plyutaba@gmail.com
Russian Federation, Moscow, 123182; Moscow, 119991

E. N. Vagner

Complex of NBICS Technologies of the NRC “Kurchatov Institute”; Department of Biotechnology, Mendeleev University of Chemical Technology of Russia

Email: plyutaba@gmail.com
Russian Federation, Moscow, 123182; Moscow 125047

I. A. Khmel

Complex of NBICS Technologies of the NRC “Kurchatov Institute”

Email: plyutaba@gmail.com
Russian Federation, Moscow, 123182

V. A. Plyuta

Complex of NBICS Technologies of the NRC “Kurchatov Institute”

Author for correspondence.
Email: plyutaba@gmail.com
Russian Federation, Moscow, 123182

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2. Fig. 1. The chemical structure of the β-ion.

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3. Fig. 2. Induction of bioluminescence of the lux biosensor E. coli MG1655 (pKatG'::lux) under the action of a β-ion. The ordinate axis shows the ratio of the luminescence value to the optical density of the culture of the lux biosensor E. coli MG1655 (pKatG'::lux) after 60 minutes (light columns) and 120 minutes (dark columns) of growth in the control (without the addition of VOCs) and under the action of 10 and 50 mmol β-ion, respectively. Hydrogen peroxide (100 mmol) was used as a positive control. All values represent average values ± standard deviations.

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4. Fig. 3. Induction of bioluminescence of the lux biosensor E. coli MG1655 (pColD'::lux) under the action of a β-ion. The ordinate axis shows the ratio of the luminescence value to the optical density of the culture of the lux biosensor E. coli MG1655 (pColD'::lux) after 60 minutes (light columns) and 120 minutes (dark columns) of growth in the control (without the addition of VOCs) and under the action of 10 and 50 mmol β-ion, respectively. Nalidixic acid (210 mmol) was used as a positive control. All values represent average values ± standard deviations.

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