Email this article CryoEM Investigation of Three-Dimentional Structure of the Stx-Converting Bacteriophage ϕ24B
- Authors: Kuznetsov A.S.1, Moiseenko A.V.2, Kulikov E.E.1, Letarov A.V.1,2
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Affiliations:
- Research Center of Biotechnology, Russian Academy of Sciences
- Lomonosov Moscow State University
- Issue: Vol 93, No 3 (2024)
- Pages: 336-339
- Section: КРАТКИЕ СООБЩЕНИЯ
- URL: https://journals.rcsi.science/0026-3656/article/view/265065
- DOI: https://doi.org/10.31857/S0026365624030095
- ID: 265065
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Abstract
A modified method for culturing, concentrating, and purifying phage ϕ24B preparations was developed. In particular, a new lysogenic phage-producing strain lacking flagella was used, induction conditions were optimized, and purification in a sucrose gradient and concentration by deposition on a Freon 113 cushion were used. Using this method, a preparation of the Stx-converting bacteriophage ϕ24B was obtained, which was suitable for direct analysis by the cryoEM method. Based on cryoEM data for this phage, the first primary three-dimensional reconstruction of its virions was performed. The structure of the phage ϕ24B tail is described. It was shown that the adsorption apparatus of this virus is represented by six thin lateral fibrils and an axial fibril located at the end of the tail. This arrangement of the tail structure is consistent with the previously proposed hypothesis based on analysis of the receptor binding proteins (RBPs) of this bacteriophage.
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About the authors
A. S. Kuznetsov
Research Center of Biotechnology, Russian Academy of Sciences
Author for correspondence.
Email: alexbluesking@gmail.com
Russian Federation, Moscow
A. V. Moiseenko
Lomonosov Moscow State University
Email: alexbluesking@gmail.com
Russian Federation, Moscow
E. E. Kulikov
Research Center of Biotechnology, Russian Academy of Sciences
Email: alexbluesking@gmail.com
Russian Federation, Moscow
A. V. Letarov
Research Center of Biotechnology, Russian Academy of Sciences; Lomonosov Moscow State University
Email: alexbluesking@gmail.com
Russian Federation, Moscow; Moscow
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