Microbial Community Composition as an Indicator of the State of Basins Located at the Sea Coast (Exemplified by the Kanda Bay, Kandalaksha Gulf, White Sea)

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Abstract

Abstract—

Formation of the Kanda Bay resulted from construction of a railway dam (1916) and subsequent isolation of the sea lagoon from the main basin of the Kandalaksha Gulf, White Sea. Decreased action of tidal flows, which mix the water column of the lagoon, altered the hydrological regime of the basin. Decreased water exchange resulted in formation of oxygen-depleted near-bottom water and to sulfide contamination. A freshwater lake was, however, preserved in the southern part of the Kanda Bay. The composition of microbial communities was studied for the near-bottom water horizons at different sides of the Kanda Bay. The oxygen regime in this layer was found to change, with increasing concentrations of sulfide and methane and active processes of sulfate reduction and methane oxidation. The composition of the microbial community changed noticeably, with lower abundance of true marine and freshwater microorganisms and development of bacteria and archaea predominant in microbial communities of anoxic water in meromictic basins. Among the microbial diversity, indicator species with increased abundance were revealed. These are archaea of the genera Methanoregula and Methanosaeta (phylum Halobacterota). The sulfur cycle microorganisms, which were the indicators of stagnant marine water, included anoxygenic phototrophic bacteria of the class Chlorobia, (Chlorobium phaeovibrioides, Pelodictyon phaeoclathratiforme), Chloroflexi of the genus Chloronema, nonsulfur purple bacteria related to the genus Rhodoferax, colorless sulfur bacteria of the family Beggiatoaceae, and sulfur oxidizers of the genus Thiobacillus. Archaea of the genus Nitrosopumilus (phylum Crenarchaeota) and bacteria of the genus Woeseia may be considered opposites to the indicator microorganisms, since they were found only in the open sea water. In our opinion, stable water exchange through the dam will result in the stable composition of the Kanda Bay microbial community, with only seasonal variations and year-to-year fluctuations. The negative scenario supports prediction of conversion of the Kanda Bay into a stratified basin with anoxic near-bottom water and the microbial community similar to that found in meromictic lakes.

About the authors

A. S. Savvichev

Winogradsky Institute of Microbiology, Federal Research Center of Biotechnology, Russian Academy of Sciences

Author for correspondence.
Email: savvichev@mail.ru
Russia, 119071, Moscow

N. A. Demidenko

Shirshov Institute of Oceanology, Russian Academy of Sciences

Email: savvichev@mail.ru
Russia, 117997, Moscow

V. V. Kadnikov

Skryabin Institute of Bioengineering, Federal Research Center of Biotechnology, Russian Academy of Sciences

Email: savvichev@mail.ru
Russia, 119071, Moscow

V. V. Belenkova

Winogradsky Institute of Microbiology, Federal Research Center of Biotechnology, Russian Academy of Sciences

Email: savvichev@mail.ru
Russia, 119071, Moscow

I. I. Rusanov

Winogradsky Institute of Microbiology, Federal Research Center of Biotechnology, Russian Academy of Sciences

Email: savvichev@mail.ru
Russia, 119071, Moscow

V. M. Gorlenko

Winogradsky Institute of Microbiology, Federal Research Center of Biotechnology, Russian Academy of Sciences

Email: savvichev@mail.ru
Russia, 119071, Moscow

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