Biodegradation of Phthalic Acid Esters by the White Rot Fungus Peniophora lycii

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Abstract

Abstract—

The ability of the white rot fungus Peniophora lycii to degrade such phthalic acid esters (PAEs) as diethyl phthalate (DEP), dibutyl phthalate (DBP), di(2-ethylhexyl) phthalate (DEHP), diisobutyl phthalate (DiBP), and n-butylbenzyl phthalate (BBP) was studied. It was shown that DEHP was most efficiently biodegraded by the fungus (over 98% on day 6 of cultivation). The residual content of DBP and DiBP in the culture liquid of the fungus at the end of cultivation (10 days) was ~17–18%. BBP turned out to be the most difficult-to-degrade compound: its residual content on day 10 of P. lycii cultivation was ~40%. DEP was resistant to fungal biodegradation, and exhibited a toxic effect at 1.5 g/L: the rate of radial growth of the fungus on agar decreased threefold compared to the control, and the amount of fungal biomass during liquid-phase deep cultivation decreased by about 1.5 times. During the cultivation of P. lycii on media with PAEs, an increase in esterase activity by about 2 times and a significant decrease (by 2–4 times) in oxidase activity was shown compared to the control medium without phthalates.

About the authors

O. S. Savinova

Bach Institute of Biochemistry, Researsch Center of Biotechnology, Russian Academy of Sciences

Email: fedorova_tv@mail.ru
Russia, 119071, Moscow

A. V. Shabaev

Bach Institute of Biochemistry, Researsch Center of Biotechnology, Russian Academy of Sciences

Email: fedorova_tv@mail.ru
Russia, 119071, Moscow

T. V. Fedorova

Bach Institute of Biochemistry, Researsch Center of Biotechnology, Russian Academy of Sciences

Author for correspondence.
Email: fedorova_tv@mail.ru
Russia, 119071, Moscow

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Copyright (c) 2023 О.С. Савинова, А.В. Шабаев, Т.В. Федорова

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