Microbial Communities Associated with the White Sea Red Algae as a Source of Xylanolytic Microorganisms

V. D. Salova; Салова В. Д.; A. M. Kholdina; Холдина А. М.; A. D. Mel’nik; Мельник А. Д.; K. S. Zayulina; Заюлина К. С.; A. G. El’cheninov; Ельченинов А. Г.; A. A. Klyukina; Клюкина А. А.; I. V. Kublanov; Кубланов И. В.

doi:10.31857/S0026365622600882

Microbial Communities Associated with the White Sea Red Algae as a Source of Xylanolytic Microorganisms

Authors: Salova V.D.¹, Kholdina A.M.¹, Mel’nik A.D.¹, Zayulina K.S.², El’cheninov A.G.², Klyukina A.A.², Kublanov I.V.¹^,2
Affiliations:
1. Faculty of Biology, Moscow State University
2. Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences
Issue: Vol 92, No 3 (2023)
Pages: 300-309
Section: EXPERIMENTAL ARTICLES
URL: https://journals.rcsi.science/0026-3656/article/view/138220
DOI: https://doi.org/10.31857/S0026365622600882
EDN: https://elibrary.ru/FXDEOK
ID: 138220

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Abstract

—Microorganisms associated with algae and able to utilize complex substrates (e.g., plant heteropolysaccharides) may be important producers of hydrolytic enzymes. The microbial communities of the red algae Corallina sp. and Phyllophora sp. sampled in the Kandalaksha Gulf basin of the White Sea were analyzed using high-throughput sequencing of the V4-variable region of the 16S rRNA gene. The dominant phyla in microbiomes of both samples were Pseudomonadota and Bacteroidota (GTDB classification, https://gtdb.ecogenomic.org/). For the Corallina sp. sample, dominance of the Vibrio, Agarivorans, and Photobacterium genera was shown, while Granulosicoccus and Aliivibrio dominated in the Phyllophora sp. sample. The analyzed red macroalgae with associated microbiota were used as an inocula to obtain microbial enrichment cultures growing on β-1,4-xylan or β-1,3-glucan (сurdlan). It was shown that, similar to environmental samples Pseudomonadota and Bacteroidota phyla representatives were prevalent in all enrichment cultures. However, unlike the environmental samples, in the enrichment cultures the dominant genera were Marinomonas, Reinekea, Polaribacter, and Pseudoalteromonas. The latter, as well as the representatives of Vibrio sp., were isolated in pure cultures for which the xylanolytic activity was shown.

Keywords

White Sea, red algae, microbial diversity, high-throughput sequencing, 16S rRNA gene, xylanolytic microorganisms

References

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