Analysis of the Key Determinants of Naphthalene Degradation by  Rhodococcus   pyridinivorans  5Ар

A. Yu. Larchenka; Ларченко А. Ю.; M. I. Mandryk; Мандрик М. И.

doi:10.31857/S0026365623600025

Analysis of the Key Determinants of Naphthalene Degradation by Rhodococcus pyridinivorans 5Ар

Authors: Larchenka A.Y.¹, Mandryk M.I.¹
Affiliations:
1. Belarusian State University
Issue: Vol 92, No 4 (2023)
Pages: 418-426
Section: EXPERIMENTAL ARTICLES
URL: https://journals.rcsi.science/0026-3656/article/view/138218
DOI: https://doi.org/10.31857/S0026365623600025
EDN: https://elibrary.ru/RIWQWF
ID: 138218

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Abstract
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Abstract

Abstract—Wild-type cells of Rhodococcus pyridinivorans 5Ар were found to be highly efficient naphthalene degraders, completely utilizing this compound (500 mg/L) after 3 days, and may be used for remediation of naphthalene-contaminated aquatic ecosystems. Inactivation of the biodegradation genes narAa (encoding the large subunit of naphthalene dioxygenase) and narB (encoding cis-naphthalene dihydrodiol dehydrogenase) resulted it the loss of ability to use naphthalene as the sole energy source, which indicated the absence in the genome of R. pyridinivorans 5Ар of the determinants responsible for alternative pathways of naphthalene oxidation. Moreover, narB inactivation resulted in accumulation of a polar colored compound (probably a product of primary naphthalene oxidation) in the medium.

Keywords

naphthalene biodegradation, Rhodococcus, biodegradation genes, growth dynamics, biodegradation efficiency, dioxygenase

About the authors

A. Yu. Larchenka

Belarusian State University

Author for correspondence.
Email: larch.alina@gmail.com
Belarus, 220030, Minsk

M. I. Mandryk

Belarusian State University

Email: larch.alina@gmail.com
Belarus, 220030, Minsk

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