Quorum Sensing in  Chromobacterium subtsugae  (Previously –  C .  violaceum ) Is Inhibited by Gamma-Lactones, the Minor Components of Eucalyptus Leaf Extract

K. S. Inchagova; Инчагова К. С.; G. K. Duskaev; Дускаев Г. К.; D. G. Deryabin; Дерябин Д. Г.

doi:10.31857/S0026365622600626

Quorum Sensing in Chromobacterium subtsugae (Previously – C. violaceum) Is Inhibited by Gamma-Lactones, the Minor Components of Eucalyptus Leaf Extract

Authors: Inchagova K.S.¹, Duskaev G.K.¹, Deryabin D.G.¹
Affiliations:
1. Federal Scientific Center for Biological Systems and Agrotechnologies, Russian Academy of Sciences
Issue: Vol 92, No 1 (2023)
Pages: 47-56
Section: ЭКСПЕРИМЕНТАЛЬНЫЕ СТАТЬИ
URL: https://journals.rcsi.science/0026-3656/article/view/138168
DOI: https://doi.org/10.31857/S0026365622600626
EDN: https://elibrary.ru/NMRUHT
ID: 138168

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Abstract

Abstract—The goal of the study was to investigate the mechanism of quorum sensing (QS) inhibition in the model bacterium Chromobacterium subtsugae 026 (previously – C. violaceum 026) by Eucalyptus viminalis Labill leaf extract, and to identify the plant-derived components that provide this biological activity. The raw extract showed pronounced anti-QS activity within a broad concentrations range which were an order of magnitude lower than required for bacterial growth suppression (the MIC₅₀/EC₅₀ ratio of 43.6). Chemical analysis of the extract revealed six small plant-derived molecules: pyrogallol, 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-1, p-cymene, 4-((1E)-3-hydroxy-1-propenyl)-2-methoxyphenol, gallic and palmitic acids, whose inhibitory effect on QS in Chromobacterium spp. has previously been shown by in vitro experiments or predicted by in silico models. In addition, minor components γ-caprolactone and γ-octanolactone, structurally similar to acylated homoserine lactones, QS autoinducers in Chromobacterium spp., were found in the extract. Chemically synthesized analogs of these molecules showed anti-QS activity in the C. subtsugae 026 bioassay (ЕС₅₀ = 354.2 and 145.6 µg/mL, respectively). Enrichment of the E. viminalis leaf extract with γ-caprolactone or γ-octanolactone significantly increased the anti-QS activity of these compositions, which was determined by isobolographic analysis as additive or superadditive effects.

Keywords

quorum sensing, Chromobacterium subtsugae, Eucalyptus viminalis, γ-caprolactone, γ-octanolactone

References

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