RESPONSES OF BARLEY OF TWO GENOTYPES TO Fusarium culmorum INFECTION

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Abstract

Fusarium culmorum is a hemibiotrophic phytopathogenic fungus that causes Fusarium head blight, Fusarium root rot and Fusarium crown rot of barley and wheat. These diseases result in reductions in yield as well as in grain contamination with mycotoxins which are dangerous to human and animal health. In this work, the early stages of the interactions between F. culmorum and barley with different resistance to root rot were studied. Barley responses were assessed by the intensity of expression of the genes PR1, PR4, LOX, PAL, NPR1, WRKY33, WRKY34 associated with pathogenesis. The state of F. culmorum in barley roots was determined by the amount of its DNA and the intensity of expression of the TRI13 gene encoding the production of trichothecene toxins by the fungus. As a result, differences in the responses of barley of two genotypes to F. culmorum colonization were established. In medium-resistant barley, in response to the pathogen attack, the PR1, PR4, and LOX genes were induced earlier than in susceptible barley. In medium-resistant barley, the expression of protective genes was increased more often than in susceptible barley compared to the control. An increase in the amount of F. culmorum DNA and the level of toxins produced by it was accompanied by suppression of the expression of the PR4, PAL, WRKY33, and WRKY34 genes in susceptible barley. In medium-resistant barley during this period, there was no suppression of protective genes; on the contrary, compared with the control, the expression of the PR1, PR4, LOX, PAL, and NPR1 genes was increased. The involvement of the studied genes in the interactions between barley of two genotypes and F. culmorum has been determined. The results obtained expand the knowledge about the molecular interactions between the parasite and the host plant in the barley – Fusarium culmorum pathosystem.

About the authors

M. I. Lebedinskii

All-Russia Research Institute for Agricultural Microbiology

Email: lebedin21@bk.ru
196608 St. Petersburg, Russia

V. Y. Shakhnazarova

All-Russia Research Institute for Agricultural Microbiology

Email: shahnazarova-v@mail.ru
196608 St. Petersburg, Russia

N. A. Vishnevskaya

All-Russia Research Institute for Agricultural Microbiology

Email: navishnevskaya@rambler.ru
196608 St. Petersburg, Russia

E. V. Borodina

All-Russia Research Institute for Agricultural Microbiology

Email: borodina_e@inbox.ru
196608 St. Petersburg, Russia

A. I. Shaposhnikov

All-Russia Research Institute for Agricultural Microbiology

Email: ai-shaposhnikov@mail.ru
196608 St. Petersburg, Russia

O. K. Strunnikova

All-Russia Research Institute for Agricultural Microbiology

Email: olgastrunnikova@rambbler.ru
196608 St. Petersburg, Russia

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