Antagonistic potential of bacterial strains of the genera Bacillus and Pseudomonas and fungi of the genus Trichoderma isolated in Uzbekistan against Phytophthora infestans

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Abstract

Studies have been carried out on strains of bacteria and fungi isolated in Uzbekistan, which can be used in the biological control of potato late blight. Testing was carried out on two strains of Phytophthora infestans — TVKT-1 and 4MSLK 26 — isolated from affected potato in the Tashkent region of Uzbekistan and in the Moscow region of Russia, respectively. In relation to these strains, the antagonistic activity of strains of fungi belonging to the genus Trichoderma and bacteria of the genera Bacillus and Pseudomonas isolated in Uzbekistan was studied. The maximum antagonistic activity was shown by the Bacillus safensis 3/11 strain, which inhibited the growth of the mycelium of the TVKT-1 strain by 61.9% and 4MSLK 26 by 50%. The strains B. licheniformis 6/25 and Pseudomonas alcaliphila 2/18 were also quite effective. All studied strains of fungi of the genus Trichoderma showed high antagonistic activity; the most active strain was T. asperellum Uz-A4, which inhibited the growth of mycelium of strains TVKT-1 and 4MSLK 26 by 77.1 and 73.1%, respectively.

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About the authors

N. Sh. Azimova

Institute of Microbiology, Academy of Sciences of the Republic of Uzbekistan

Author for correspondence.
Email: azimovanodira@mail.ru
Uzbekistan, 100128, Tashkent

H. M. Khamidova

Institute of Microbiology, Academy of Sciences of the Republic of Uzbekistan

Email: khamidovakh@mail.ru
Uzbekistan, 100128, Tashkent

I. M. Khalilov

Institute of Microbiology, Academy of Sciences of the Republic of Uzbekistan

Email: ilkhom2002@yahoo.com
Uzbekistan, 100128, Tashkent

S. N. Elansky

M.V. Lomonosov Moscow State University; Peoples’ Friendship University of Russia

Email: snelansky@mail.ru
Russian Federation, 119991, Moscow; 117198, Moscow

E. M. Chudinova

Peoples’ Friendship University of Russia

Email: chudiel@mail.ru
Russian Federation, 117198, Moscow

H. H. Karimov

Institute of Microbiology, Academy of Sciences of the Republic of Uzbekistan

Email: karimov_h_kh@mail.ru
Uzbekistan, 100128, Tashkent

K. S. Mamanazarova

Institute of Botany of the Academy of Sciences of the Republic of Uzbekistan

Email: karomat.3005@mail.ru
Uzbekistan, 100125, Tashkent

F. B. Kobilov

Institute of Microbiology, Academy of Sciences of the Republic of Uzbekistan

Email: bozorovich02@mail.ru
Uzbekistan, 100128, Tashkent

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2. Fig. 1. Genomic DNA of Trichoderma strains: 1 — Trichoderma sp. Uz-A21; 2 — Trichoderma sp. Uz-A4; A — genomic DNA; M — marker of genomic DNA; B — PCR product of ITS-region of Trichoderma strains; M — DNA marker 100 bp long.

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3. Fig. 2. Antagonistic activity of bacteria in relation to Phytophthora infestans TVKT-1.

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4. Fig. 3. Antagonistic activity of bacteria in relation to Phytophthora infestans 4 M With LC 26.

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5. Fig. 4. Antagonistic properties of fungi of the genus Trichoderma in relation to Phytophthora infestans TVKT-1 and Ph. infestans 4MSLK 26 (Day 6).

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6. Fig. 5. Antagonistic activity of fungi of the genus Trichoderma in relation to Phytophthora infestans TVKT-1 and Ph. infestans 4MSLK 26 (Day 10).

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