Aspergillus niger АМ1 strain as causative agent for oil and petroleum products biodegradation

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Abstract

The biodegradation of oils by the strain Aspergillus niger AM1 VKM F-4815D was studied. Visual observation and gas chromatography-mass spectrometry showed that oil is subject to partial destruction, but cannot serve as the only source of carbon — the culture medium must contain glucose. An interesting fact is the change in consistency and hardening of oil under the influence of A. niger. This allows us to consider the possibility of using the strain for the bioremediation of soils and waters contaminated with oil. No less interesting is that, even earlier, the ability of the strain to metabolize a number of toxic phosphorus compounds, including even white and red phosphorus, was established. However, most organic solvents have a noticeable toxic effect, inhibiting growth in the presence of glucose and not becoming carbon sources in the absence of glucose.

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About the authors

A. Z. Mindubaev

Kazan National Research Technological University

Author for correspondence.
Email: mindubaev@iopc.ru
Russian Federation, 420015, Kazan

E. V. Babynin

Kazan (Volga Region) Federal University

Email: edward.b67@mail.ru
Russian Federation, 420008, Kazan

V. M. Babaev

Institute of Organic and Physical Chemistry named after. A.E. Arbuzov Kazan Scientific Center RAS

Email: babaev-84@mail.ru
Russian Federation, 420088, Kazan

V. V. Tutuchkina

Institute of Organic and Physical Chemistry named after. A.E. Arbuzov Kazan Scientific Center RAS

Email: tutuchkinav@mail.ru
Russian Federation, 420088, Kazan

S. T. Minzanova

Institute of Organic and Physical Chemistry named after. A.E. Arbuzov Kazan Scientific Center RAS

Email: minzanova@iopc.ru
Russian Federation, 420088, Kazan

L. G. Mironova

Institute of Organic and Physical Chemistry named after. A.E. Arbuzov Kazan Scientific Center RAS

Email: mironoval1963@gmail.com
Russian Federation, 420088, Kazan

Yu. V. Karaeva

Kazan National Research Technological University

Email: julieenergy@list.ru
Russian Federation, 420015, Kazan

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2. Fig. 1. Oil samples inoculated with the Aspergillus niger strain AM1: G – glucose; NK – negative control; N-Sh – oil of the Novo-Sheshminskoye field; P – oil of the Pervomaisky field. Mycelium growth is noticeable only in the presence of glucose. The picture was taken on the 36th day after sowing.

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3. Fig. 2. Decrease in the amount of oil components from the Pervomaisky field as a result of biodegradation by the fungus Aspergillus niger AM1.

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4. Fig. 3. Growth of Aspergillus niger AM1 on acetonitrile: A – acetonitrile; G – glucose; NK – negative control. The blue color of the oxidized 2,6-dichlorophenolindophenol indicates the absence of metabolic processes. The absence of color in the reduced dye indicates the vital activity of A. niger. The picture was taken on the 26th day after sowing.

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