The Peculiarities of the Mycobiota Formation on the Saint Petersburg Stone Monuments Based on Metagenomics and Cultural Data

K. V. Sazanova; Сазанова К. В.; M. S. Zelenskaya; Зеленская М. С.; O. A. Pavlova; Павлова О. А.; D. Yu. Vlasov; Власов Д. Ю.

doi:10.31857/S0026364823050070

The Peculiarities of the Mycobiota Formation on the Saint Petersburg Stone Monuments Based on Metagenomics and Cultural Data

Authors: Sazanova K.V.¹^,2^,3, Zelenskaya M.S.², Pavlova O.A.⁴, Vlasov D.Y.¹^,2
Affiliations:
1. Komarov Botanical Institute of the Russian Academy of Sciences
2. St. Petersburg State University
3. Archive of the Russian Academy of Sciences, St. Petersburg Branch
4. Beagle LTD Company
Issue: Vol 57, No 5 (2023)
Pages: 321-331
Section: БИОРАЗНООБРАЗИЕ, СИСТЕМАТИКА, ЭКОЛОГИЯ
URL: https://journals.rcsi.science/0026-3648/article/view/138078
DOI: https://doi.org/10.31857/S0026364823050070
EDN: https://elibrary.ru/YECIMP
ID: 138078

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Abstract

Fungi play an important role in colonization and biodeterioration of stone monuments in the open air. This study significantly complements the data on fungal diversity in lithobiotic communities through the use of metagenomic analysis. It was shown that the mycobiota of tombstones in the historical center of St. Petersburg has a complex structure. There are different complexes of species, which have various origins and ways of getting to the monuments. The complex of dominant fungi in SABs on marble and granite in St. Petersburg is formed by dark-colored micromycetes. At the species level, the absolute dominant in all samples was the Knufia karalitana according to metagenomic data and Aureobasidium pullulans according to cultural data. The use of two methodological approaches indicates the expediency of combining culture-based and molecular genetics methods, which make it possible to obtain the most more complete picture of the formation of lithobiotic communities. The season and the type of biofilm have a key importance for the abundance and diversity of micromycetes on stone surface. It was shown that type of rock has a minimal importance for the fungal diversity on stone monument.

Keywords

cultivation and metagenomic methodologies, cultural heritage, fungal diversity, microbial community, microfungi, subaerial biofilms

References

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