Temperature influence on the stability of the precursor cluster of the thermolysin crystal
- Autores: Kordonskaya Y.1, Timofeev V.2, Marchenkova M.2,3, Pisarevsky Y.2, Silvestrova S.4, Dyakova Y.1, Kovalchuk M.1,2
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Afiliações:
- National Research Centre “Kurchatov Institute”
- NRC “Kurchatov Institute”
- Southern Federal University
- The Loginov Moscow Clinical Scientific Center Moscow Health Department
- Edição: Volume 69, Nº 4 (2024)
- Páginas: 694-699
- Seção: CRYSTAL GROWTH
- URL: https://journals.rcsi.science/0023-4761/article/view/264435
- DOI: https://doi.org/10.31857/S0023476124040165
- EDN: https://elibrary.ru/XBSOKY
- ID: 264435
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Resumo
We used the molecular dynamics method to assess the stability of the precursor-cluster (hexamer) of thermolysin crystal over a wide range of temperatures (10–90°C). The simulation results showed that as the temperature increases, the stability of the hexamer, in general, decreases, however, the hexamer does not dissociate at any of the considered temperatures. At a temperature of 60°C, an increase in the stability of the hexamer was observed. This value is close to the temperature of maximum enzymatic activity of thermolysin (70°C). Based on the analysis of the results, it was assumed that the crystallization of thermolysin could be carried out at 60°C.
Sobre autores
Y. Kordonskaya
National Research Centre “Kurchatov Institute”
Autor responsável pela correspondência
Email: yukord@mail.ru
Rússia, Moscow
V. Timofeev
NRC “Kurchatov Institute”
Email: yukord@mail.ru
Shubnikov Institute of Crystallography of Kurchatov Complex of Crystallography and Photonics
Rússia, MoscowM. Marchenkova
NRC “Kurchatov Institute”; Southern Federal University
Email: yukord@mail.ru
Shubnikov Institute of Crystallography of Kurchatov Complex of Crystallography and Photonics; The Smart Materials Research Institute
Rússia, Moscow; Rostov-on-DonY. Pisarevsky
NRC “Kurchatov Institute”
Email: yukord@mail.ru
Shubnikov Institute of Crystallography of Kurchatov Complex of Crystallography and Photonics
Rússia, MoscowS. Silvestrova
The Loginov Moscow Clinical Scientific Center Moscow Health Department
Email: yukord@mail.ru
Rússia, Moscow
Y. Dyakova
National Research Centre “Kurchatov Institute”
Email: yukord@mail.ru
Rússia, Moscow
M. Kovalchuk
National Research Centre “Kurchatov Institute”; NRC “Kurchatov Institute”
Email: yukord@mail.ru
Shubnikov Institute of Crystallography of Kurchatov Complex of Crystallography and Photonics
Rússia, Moscow; MoscowBibliografia
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