Email this article Temperature influence on the stability of the precursor cluster of the thermolysin crystal
- Authors: Kordonskaya Y.V.1, Timofeev V.I.2, Marchenkova M.А.2,3, Pisarevsky Y.V.2, Silvestrova S.Y.4, Dyakova Y.A.1, Kovalchuk M.V.1,2
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Affiliations:
- National Research Centre “Kurchatov Institute”
- NRC “Kurchatov Institute”
- Southern Federal University
- The Loginov Moscow Clinical Scientific Center Moscow Health Department
- Issue: Vol 69, No 4 (2024)
- Pages: 694-699
- Section: CRYSTAL GROWTH
- URL: https://journals.rcsi.science/0023-4761/article/view/264435
- DOI: https://doi.org/10.31857/S0023476124040165
- EDN: https://elibrary.ru/XBSOKY
- ID: 264435
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Abstract
We used the molecular dynamics method to assess the stability of the precursor-cluster (hexamer) of thermolysin crystal over a wide range of temperatures (10–90°C). The simulation results showed that as the temperature increases, the stability of the hexamer, in general, decreases, however, the hexamer does not dissociate at any of the considered temperatures. At a temperature of 60°C, an increase in the stability of the hexamer was observed. This value is close to the temperature of maximum enzymatic activity of thermolysin (70°C). Based on the analysis of the results, it was assumed that the crystallization of thermolysin could be carried out at 60°C.
About the authors
Y. V. Kordonskaya
National Research Centre “Kurchatov Institute”
Author for correspondence.
Email: yukord@mail.ru
Russian Federation, Moscow
V. I. Timofeev
NRC “Kurchatov Institute”
Email: yukord@mail.ru
Shubnikov Institute of Crystallography of Kurchatov Complex of Crystallography and Photonics
Russian Federation, MoscowM. А. Marchenkova
NRC “Kurchatov Institute”; Southern Federal University
Email: yukord@mail.ru
Shubnikov Institute of Crystallography of Kurchatov Complex of Crystallography and Photonics; The Smart Materials Research Institute
Russian Federation, Moscow; Rostov-on-DonY. V. Pisarevsky
NRC “Kurchatov Institute”
Email: yukord@mail.ru
Shubnikov Institute of Crystallography of Kurchatov Complex of Crystallography and Photonics
Russian Federation, MoscowS. Y. Silvestrova
The Loginov Moscow Clinical Scientific Center Moscow Health Department
Email: yukord@mail.ru
Russian Federation, Moscow
Y. A. Dyakova
National Research Centre “Kurchatov Institute”
Email: yukord@mail.ru
Russian Federation, Moscow
M. V. Kovalchuk
National Research Centre “Kurchatov Institute”; NRC “Kurchatov Institute”
Email: yukord@mail.ru
Shubnikov Institute of Crystallography of Kurchatov Complex of Crystallography and Photonics
Russian Federation, Moscow; MoscowReferences
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