Влияние факторов свертывания на свойства адсорбционных пленок фибрина

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Abstract

Образование пленки фибрина на месте порезов и ран – сложный биохимический процесс, в котором, помимо основных компонентов – фибриногена и тромбина – участвуют также другие ферменты и белки. Адсорбционные пленки, полученные из раствора, содержащего факторы VIII, XIIIа, фактор Виллебранда и фибронектин, имеют ряд отличий по сравнению с пленками фибриногена и пленками фибрина, полученными из раствора, содержащего только фибриноген и тромбин. Динамическая поверхностная упругость превышает соответствующие значения для фибриногена (80 мН/м и 55 мН/м соответственно), однако оказывается ниже значений для фибрина, полученного из чистых компонентов (115 мН/м). Поверхностные давления для адсорбционных пленок, полученных из гемостатического клея (27 мН/м), оказываются выше значений для обеих рассмотренных ранее систем (14 мН/м). Это связано с существенными изменениями в морфологии полученных пленок, которая была оценена с помощью микроскопии при угле Брюстера и сканирующей электронной микроскопии.

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About the authors

О. Ю. Миляева

Санкт-Петербургский государственный университет

Author for correspondence.
Email: o.milyaeva@spbu.ru

Институт химии

Russian Federation, Университетский проспект, 26, Санкт-Петербург, 198504

А. Р. Рафикова

Санкт-Петербургский государственный университет

Email: o.milyaeva@spbu.ru

Институт химии

Russian Federation, Университетский проспект, 26, Санкт-Петербург, 198504

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Supplementary files

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2. Fig. 1. Kinetic dependences of dynamic surface elasticity (a) and dynamic surface tension (b) of fibrinogen solutions with a concentration of 3 · 10-7 M (1); solutions containing fibrinogen with a concentration of 3 · 10-7 M and thrombin with a concentration of 300 U/l (2); solutions of mixtures of fibrinogen-containing and thrombin‑containing components of the glue “Cryophyte”, taken in the proportions 10 : 1 (3), 5 : 1 (4), 2 : 1 (5), 1 : 1 (6).

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3. Fig. 2. Dependences of dynamic surface elasticity on the surface pressure of solutions of fibrinogen with a concentration of 3 · 10-7 M (1); solutions containing fibrinogen with a concentration of 3 · 10-7 M and thrombin with a concentration of 300 U/l (2); solutions of mixtures of fibrinogen-containing and thrombin- containing components of the glue “Cryophyte”, taken together 10 : 1 (3), 5 : 1 (4), 2 : 1 (5), 1 : 1 (6).

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4. Fig. 3. Compression isotherms for fibrinogen solutions with a concentration of 3 · 10-7 M (1); solutions containing fibrinogen with a concentration of 3 · 10-7 M and thrombin with a concentration of 300 U/l (2); solutions of mixtures of fibrinogen-containing and thrombin- containing components of the glue “Cryophyte”, taken together 10 : 1 (3), 5 : 1 (4), 2 : 1 (5), 1 : 1 (6).

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5. Fig. 4. Kinetic dependences of the ellipsometric angle Δ (a) and kinetic dependences of the film thickness (b) for fibrinogen solutions with a concentration of 1 · 10-7 M (1) and solutions of mixtures of fibrinogen-containing and thrombin‑containing components of Cryophyte glue, taken in the following ratios 10 : 1 (2), 5 : 1 (3), 2 : 1 (4), 1 : 1 (5).

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6. Fig. 5. SEM images of fibrin films obtained from solutions of mixtures of fibrinogen-containing and thrombin‑containing components of the Cryophyte glue, taken in ratios of 5:1 (a, b), 2: 1 (c, d), 1 :1 (e, e).

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7. Fig. 6. Images of fibrin films obtained by Brewster's angle microscopy for solutions of mixtures of fibrinogen-containing and thrombin-containing components of Cryophyte glue in a ratio of 1 : 1. The image (a) corresponds to an equilibrium film, the image (b) – after the imposition of a small mechanical disturbance, the image (c) ‑ the repeated imposition of a small mechanical disturbance.

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8. Fig. 7. AFM image of a fibrin film obtained from a solution of a mixture of fibrinogen with a concentration of 3 · 10-7 M and thrombin with a concentration of 100 U/l (a) and 300 units/l (b).

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