Kriogranulirovanie molochnokislykh bakteriy s vysokim pokazatelem krioustoychivosti

This work covers the problem of keeping of stability and survival rate of Lactococcus lactis lactis and Lactococcus lactis cremoris lactobacilli. The influence of the suspension density of lactobacilli on their survival rate, on cryogranules as well as on cryogranules that were subject to cool dehumidification was studied. The factors influencing the survival rate and lactobacilli’s activity were revealed. The investigations resulted in accumulation of experimental data on the lactobacilli survival rate and activity at cryogranulation and their using in cryogranulating form. It was specified that the suspension density of lactobacilli before cryogranulation impacts on the survival rate to a very little degree after cryogranulation, but it impacts greatly on the granules state, particularly after cool dehumidification. Also the suspension density impacts on the granule colour: the higher the density, the darker the colour of the granule. It was noted that the cryogranules when granulating without additional components have a high level of survival rate, but to make further cool dehumidification it was necessary to use sucrose or maltose solutions. An acidforming capability of cryogranulated lactobacilli didn’t change during the cryogranulation process. So, it was shown that the Lactococcus lactis lactis and Lactococcus lactis cremoris cultures yielded well to cryogranulation in liquid nitrogen and had a high survival rate and kept an acidforming capability as well.

лактококки, криогранулирование, плотность суспензии