The effect of reactive oxygen and nitrogen species generated by electric discharges on the primary structure of protein and biological objects

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Abstract

The effect of reactive oxygen and nitrogen species generated by cold plasma of corona electric discharge, pulsed radiation of hot plasma and UV radiation of low-pressure mercury lamp on albumin protein, amino acids tyrosine, tryptophan, phenylalanine and Gram-positive, Gram-negative bacteria and micromycetes was investigated. The degree of degradation of all samples was assessed by fluorescence reduction and microbial survival by CFU value after culturing. It was found that fluorescence is determined by the primary protein structure, while microbial survival by second and higher order structures.

About the authors

I. M. Piskarev

Skobeltsyn Institute of Nuclear Physics (SINP MSU), M.V. Lomonosov Moscow State University

Email: i.m.piskarev@gmail.com
Moscow, Russia

I. P. Ivanova

Lobachevsky State University of Nizhny Novgorod

Email: ivanova.ip@mail.ru
Nizhny Novgorod, Russia

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