Email this article Phosphohydrolases in Turbellaria Phagocata sibirica
- Authors: Burenina E.A.1, Zhukovskaya M.I.2
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Affiliations:
- Federal Scientific Center of the East Asia Terrestrial Biodiversity
- Sechenov Institute of Evolutionary Physiology and Biochemistry
- Issue: Vol 55, No 4 (2019)
- Pages: 277-283
- Section: Comparative and Ontogenic Biochemistry
- URL: https://journals.rcsi.science/0022-0930/article/view/159775
- DOI: https://doi.org/10.1134/S0022093019040033
- ID: 159775
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Abstract
Activity and properties of two phosphohydrolases, nucleoside diphosphatase (NDPase) and 5′-nucleotidase, were assayed in subcellular fractions of turbellaria Phagocata sibirica Sabussov (Turbellaria, Planariidae). The highest activity of N D Pase was observed in microsomes while that of 5′-nucleotidase in mitochondria and cytosolic fractions (12 000 and 105 000 g). The correlation between the rates of the relevant enzymatic reactions and concentrations of substrates and metal ions as well as the impact of various effectors and divalent cations (Mg2+, Mn2+, Ca2+, Zn2+, Cu2+) on phosphohydrolase activities were studied.
About the authors
E. A. Burenina
Federal Scientific Center of the East Asia Terrestrial Biodiversity
Author for correspondence.
Email: burenina@ibss.dvo.ru
Russian Federation, Vladivostok
M. I. Zhukovskaya
Sechenov Institute of Evolutionary Physiology and Biochemistry
Email: burenina@ibss.dvo.ru
Russian Federation, St. Petersburg
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