nm-Test – An Improved Version of the Alpha-Test in Yeast Saccharomyces cerevisiae with a Higher Sensitivity to Genotoxic Factors

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Abstract

One of the urgent problems of genetic toxicology is the development of new and improvement of existing test systems for the detection and assessment of the level of mutagenic and carcinogenic activity of various factors. A unique position among the known test systems belongs to the alpha test, that is based on the life cycle of the yeast Saccharomyces cerevisiae. Its main difference from existing test systems is the ability to detect a wide range of genetic events, such as gene mutations, conversion, recombination, loss of the right arm and the entire III chromosome, as well as primary lesions to the genetic material before they are eliminated by repair systems. Here, we proposed and tested a genetic modification of tester strains that increased the sensitivity and efficiency of the test system. Our results allowed us to conclude that the proposed modification of the tester strain led to the sensitivity increase of the test system by an order of magnitude.

About the authors

E. I. Stepchenkova

Vavilov Institute of General Genetics, Saint-Petersburg Branch, Russian Academy of Sciences; Saint Petersburg State University, Department of Genetics and Biotechnology

Author for correspondence.
Email: stepchenkova@gmail.com
Russia, 199034, Saint-Petersburg; Russia, 199034, Saint Petersburg

Yu. V. Andreychuk

Vavilov Institute of General Genetics, Saint-Petersburg Branch, Russian Academy of Sciences

Email: stepchenkova@gmail.com
Russia, 199034, Saint-Petersburg

D. V. Afanasova

Saint Petersburg State University, Department of Genetics and Biotechnology

Email: stepchenkova@gmail.com
Russia, 199034, Saint Petersburg

S. P. Zadorsky

Vavilov Institute of General Genetics, Saint-Petersburg Branch, Russian Academy of Sciences; Saint Petersburg State University, Department of Genetics and Biotechnology

Email: stepchenkova@gmail.com
Russia, 199034, Saint-Petersburg; Russia, 199034, Saint Petersburg

S. G. Inge-Vechtomov

Vavilov Institute of General Genetics, Saint-Petersburg Branch, Russian Academy of Sciences; Saint Petersburg State University, Department of Genetics and Biotechnology

Email: stepchenkova@gmail.com
Russia, 199034, Saint-Petersburg; Russia, 199034, Saint Petersburg

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Copyright (c) 2022 Е.И. Степченкова, Ю.В. Андрейчук, Д.В. Афанасова, С.П. Задорский, С.Г. Инге-Вечтомов

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