Effect of Chronic Radiation Exposure on Human microRNA Expression

M. A. Yanishevskaya; Янишевская М. А.; E. A. Blinova; Блинова Е. А.; A. V. Akleyev; Аклеев А. В.

doi:10.31857/S0016675823100156

Effect of Chronic Radiation Exposure on Human microRNA Expression

Authors: Yanishevskaya M.A.¹, Blinova E.A.¹^,2, Akleyev A.V.¹^,2
Affiliations:
1. Urals Research Center for Radiation Medicine
2. Chelyabinsk State University
Issue: Vol 59, No 10 (2023)
Pages: 1171-1178
Section: ГЕНЕТИКА ЧЕЛОВЕКА
URL: https://journals.rcsi.science/0016-6758/article/view/140392
DOI: https://doi.org/10.31857/S0016675823100156
EDN: https://elibrary.ru/ZWDVWN
ID: 140392

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Abstract
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Abstract

Relative expression of mature microRNA assessed in peripheral blood cells in persons chronically exposed to low-dose radiation in the long-term period. The study included people exposed in the 1950s on the Techa River (Southern Urals, Russia). The cumulative red bone marrow doses (RBM) of the persons in the main study group (33 persons) ranged from 77.7 to 2869.8 mGy (mean value – 698.5 mGy). The comparison group consisted of 30 people with RBM dose not exceeding 70 mGy over their lifetime. Reverse transcription of RNA samples was performed using specific stem-loop primers (“stem-loop”). Changes in the relative content of microRNA were assessed by real-time PCR on the CFX96 detection system (BioRad, USA). Statistical analysis of the results was performed using Mann–Whitney U-criterion. A significant increase in hsa-miR-125b, hsa-miR-181a, and hsa-miR-16-5p content was detected in exposed people after 60 years, and their expression was found to depend on the RBM dose.

Keywords

microRNA, expression, PCR, the Techa River, chronic exposure, radiation exposure.

References

Friedman R.C., Farh K.K., Burge C.B. et al. Most mammalian mRNAs are conserved targets of microRNAs // Genome Res. 2009. V. 19. № 1. P. 92–105. https://doi.org/10.1101/gr.082701.108
Mao A., Liu Y., Zhang H. et al. microRNA expression and biogenesis in cellular response to ionizing radiation // DNA and Cell Biology. 2014. V. 33. № 10. P. 667–679. https://doi.org/10.1089/dna.2014.2401
Pashaei E., Pashaei E., Ahmady M. et al. Meta-analysis of miRNA expression profiles for prostate cancer recurrence following radical prostatectomy // PLoS One. 2017. V. 12. № 6. https://doi.org/10.1371/journal.pone.0179543
Adhami M., Haghdoost A.A., Sadeghi B. et al. Candidate miRNAs in human breast cancer biomarkers: A systematic review // Breast Cancer. 2018. V. 25. № 2. P. 198–205. https://doi.org/10.1007/s12282-017-0814-803
Pardini B., De Maria D., Francavilla A. et al. MicroRNAs as markers of progression in cervical cancer: A systematic review // BMC Cancer. 2018. V. 18. № 1. P. 696. https://doi.org/10.1186/s12885-018-4590-4
Shao C., Yang F., Qin Z. et al. The value of miR-155 as a biomarker for the diagnosis and prognosis of lung cancer: A systematic review with meta-analysis // BMC Cancer. 2019. V. 19. № 1. P. 1103. https://doi.org/10.1186/s12885-019-6297-6
Ромакина В.В., Жиров И.В., Насонова С.Н. и др. МикроРНК как биомаркеры сердечно-сосудистых заболеваний // Кардиология. 2018. Т. 58. № 1. С. 66–71. https://doi.org/10.18087/cardio.2018.1.10083
Otsuka M., Kishikawa T., Yoshikawa T. et al. MicroRNAs and liver disease // J. Hum. Genet. 2016. V. 62. P. 75–80. https://doi.org/10.1038/jhg.2016.53
Zhang Y., Jia Y., Zheng R. et al. Plasma microRNA-122 as a biomarker for viral-, alcohol-, and chemicalrelated hepatic diseases // Clin. Chem. 2010. V. 56. № 12. P. 1830–1838. https://doi.org/10.1373/clinchem.2010.147850
Metheetrairut C., Slack F.J. MicroRNAs in the ionizing radiation response and in radiotherapy // Curr. Op. in Genet. & Development. 2013. V. 23. № 1. P. 2–19. https://doi.org/10.1016/j.gde.2013.01.002
Jacob N.K., Cooley J.V., Yee T.N. et al. Identification of sensitive serum microRNA biomarkers for radiation biodosimetry // PLoS One. 2013. V. 8. № 2. https://doi.org/10.1371/journal.pone.0057603
Chaudhry M.A., Omaruddin R.A., Kreger B. et al. MicroRNA responses to chronic or acute exposures to low dose ionizing radiation // Mol. Biol. Reports. 2012. V. 39. № 7. P. 7549–7558. https://doi.org/10.1007/s11033-012-1589-9
Simone N.L., Soule B.P., Ly D. et al. Ionizing radiation-induced oxidative stress alters miRNA expression // PLoS One. 2009. V. 4. https://doi.org/10.1371/journal.pone.0006377
Shin S., Cha H.J., Lee E.M. et al. Alteration of miRNA profiles by ionizing radiation in A549 human non-small cell lung cancer cells // Int. J. Oncol. 2009. V. 35. № 1. P. 81–86.
Chaudhry M.A. Real-time PCR analysis of microRNA expression in ionizing radiation-treated cells // Cancer Biother. Radiopharm. 2009. V. 24. № 1. P. 49–56. https://doi.org/10.1089/cbr.2008.0513
Song M., Xie D., Gao S. et al. A biomarker panel of radiation-upregulated miRNA as signature for ionizing radiation exposure // Life (Basel). 2020. V. 10. № 12. P. 361. https://doi.org/10.3390/life10120361
Templin T., Paul S., Amundson S.A. et al. Radiation-induced microRNA expression changes in peripheral blood cells of radiotherapy patients // Int. J. of Rad. Oncol., Biol., Physics. 2011. V. 80. № 2. P. 54957. https://doi.org/10.1016/j.ijrobp.2010.12.061
Weidhaas I., Babar S.M., Nallur P. et al. MicroRNAs as potential agents to alter resistance to cytotoxic anticancer therapy // Cancer Res. 2007. V. 67. № 23. P. 11111–11116.
Maes O.C., An J., Sarojini H. et al. Changes in MicroRNA expression patterns in human fibroblasts after low-LET radiation // J. Cell Biochem. 2008. V. 105. № 3. P. 824–834. https://doi.org/10.1002/jcb.21878
Templin T., Paul S., Amundson S.A. et al. Radiation-induced micro-RNA expression changes in peripheral blood cells of radiotherapy patients // Int. J. Rad. Oncol. Biol. Phys. 2011. V. 80. № 2. P. 549–557. https://doi.org/10.1016/j.ijrobp.2010.12.061
Lacombe J., Sima C., Amundson S.A., Zenhausern F. Candidate gene biodosimetry markers of exposure to external ionizing radiation in human blood: A systematic review // PLoS One. 2018. V. 13. № 6. https://doi.org/10.1371/journal.pone.0198851
Jacob N.K., Cooley J.V., Yee T.N. et al. Identification of sensitive serum microRNA biomarkers for radiation biodosimetry // PLoS One. 2013. V. 8. № 2. https://doi.org/10.1371/journal.pone.0057603
Chen G., Zhu W., Shi D. et al. MicroRNA-181a sensitizes human malignant glioma U87MG cells to radiation by targeting Bcl-2 // Oncol. Reports. 2010. V. 23. № 4. P. 997–1003. https://doi.org/10.3892/or_00000725
Beer L., Seemann R., Ristl R. et al. High dose ionizing radiation regulates micro RNA and gene expression changes in human peripheral blood mononuclear cells // BMC Genomics. 2014. V. 15. P. 814. https://doi.org/10.1186/1471-2164-15-814
SunY., Hawkins P.G., Bi N. et al. Serum microRNA signature predicts response to high-dose radiation therapy in locally advanced non-small cell lung cancer // Int. J. of Rad. Oncol., Biol., Physics. 2018. V. 100. № 1. P. 107–114. https://doi.org/10.1016/j.ijrobp.2017.08.039
Силкин C.C., Крестинина Л.Ю., Старцев В.Н. и др. Уральская когорта аварийно-облученного населения // Медицина экстрем. ситуаций. 2019. Т. 21. № 3. С. 393–402.
Degteva M.O., Napier B.A., Tolstykh E.I. et al. Enhancements in the Techa River dosimetry system: TRDS-2016D code for reconstruction of deterministic estimates of dose from environmental exposures // Health Physics. 2019. V. 117. № 4. P. 378–387. https://doi.org/10.1097/HP.0000000000001067
Livak K.J., Schmittgen T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) // Methods. 2001. V. 25. № 4. P. 402–408. https://doi.org/10.1006/meth.2001.1262
Riolo G., Cantara S., Marzocchi C., Ricci C. miRNA targets: From prediction tools to experimental validation // Meth. and Protocols. 2020. V. 4. № 1. https://doi.org/10.3390/mps4010001
Stroynowska-Czerwinska A., Fiszer A., Krzyzosiak W.J. The panorama of miRNA-mediated mechanisms in mammalian cells // Cell. and Mol. Life Sci. 2014. V. 71. № 12. P. 2253–2270. https://doi.org/10.1007/s00018-013-1551-6
Aqeilan R.I., Calin G.A., Croce C.M. miR-15a and miR-16-1 in cancer: discovery, function and future perspectives // Cell Death Differ. 2010. V. 17. № 2. P. 215–220. https://doi.org/10.1038/cdd.2009.69
Chen G., Zhu W., Shi D. et al. MicroRNA-181a sensitizes human malignant glioma U87MG cells to radiation by targeting Bcl-2 // Oncol. Reports. 2010. V. 23. № 4. P. 997–1003. https://doi.org/10.3892/or_00000725
Махоткин М.А., Чеботарев Д.А., Тютякина М.Г. и др. Роль микроРНК в развитии радиорезистентности клеток рака предстательной железы (экспериментальное исследование) // Онкоурология. 2021. Т. 17. № 4. С. 85–93. https://doi.org/10.17650/1726-9776-2021-17-4-85-93
Шуленина Л.В., Михайлов В.Ф., Васильева И.М. и др. Профили экспрессии генов и некодирующих РНК в биоптатах тканей и клетках крови пациентов с разной патологией после радиационного воздействия // Вестник ВГУ. Серия: Химия. Биология. Фармация. 2019. № 1. С. 103–109.
Yang D., Zhan M., Chen T. et al. miR-125b-5p enhances chemotherapy sensitivity to cisplatin by down-regulating Bcl2 in gallbladder cancer // Sci. Rep. 2017. V. 7. https://doi.org/10.1038/srep43109
Никифоров В.С., Блинова Е.А., Аклеев А.В. Транскрипционная активность генов клеточного цикла и апоптоза у хронически облученных лиц, имеющих повышенную частоту TCR-мутантных лимфоцитов // Радиация и риск (Бюлл. Нац. рад.-эпидем. регистра). 2020. Т. 29. № 2. С. 89–100.