Study of  Myb114  Gene Polymorphism in the Cole Crops ( Brassica oleracea  L.) in Connection with Anthocyanin Biosynthesis Regulation Based on Comparison with the MYB Factors of Vegetable Nightshades (Solanaceae)

D. A. Fateev; Фатеев Д. А.; F. A. Berensen; Беренсен Ф. А.; A. M. Artemyeva; Артемьева А. М.; O. G. Babak; Бабак О. Г.; K. K. Yatsevich; Яцевич К. К.; E. V. Drozd; Дрозд Е. В.; A. V. Kilchevsky; Кильчевский А. В.

doi:10.31857/S0016675823010046

Study of Myb114 Gene Polymorphism in the Cole Crops (Brassica oleracea L.) in Connection with Anthocyanin Biosynthesis Regulation Based on Comparison with the MYB Factors of Vegetable Nightshades (Solanaceae)

Authors: Fateev D.A.¹, Berensen F.A.¹, Artemyeva A.M.¹, Babak O.G.², Yatsevich K.K.², Drozd E.V.², Kilchevsky A.V.²
Affiliations:
1. Federal Research Center Vavilov All-Russian Institute of Plant Genetic Resources (VIR)
2. Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
Issue: Vol 59, No 1 (2023)
Pages: 39-49
Section: ГЕНЕТИКА РАСТЕНИЙ
URL: https://journals.rcsi.science/0016-6758/article/view/134500
DOI: https://doi.org/10.31857/S0016675823010046
EDN: https://elibrary.ru/CLOYTD
ID: 134500

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Abstract

In order to study a regulation mechanism for one of the most ancient processes of anthocyanin biosynthesis in plants based on comparison of R2R3-MYB genes in Solanaceae (S. lycopersicum: An1, An2; S. melongena: Myb1; C. annuum: Myb113-like1 и Myb113-like2) and Brassicaeae crops, a search for orthologous sequences in Brassica oleracea was performed. A sequence encoding MYB114 TF was found to be the closest in a nucleotide structure to the previously investigated genes in Solanaceae. Primers were selected and a comparative analysis of Myb114 gene sequences was performed in B. oleracea varieties (white cabbage, red cabbage, kale, cauliflower, broccoli, and kohlrabi) with a wide intraspecies diversity by the anthocyanin accumulation in leaves and various food organs: heads, curds and stems. Alignment of Myb114 sequences revealed a number of gene polymorphisms that closely correlate with high anthocyanin accumulation in B. oleracea leaves, including 3 SNPs in exon regions and a 271 bp deletion in the 1st intron. The identified SNPs lead to the replacement of two amino acids located in the region of DNA-binding domains, which leads to a change in the binding efficiency of this transcription factor with the promoters of structural biosynthesis genes and a decrease in their expression level in the forms without anthocyanin accumulation in leaves compared with the forms with their accumulation in vegetative plant parts. A molecular marker MYB114.2 has been proposed that may be used for the identification of headed cabbages and kales with high/low anthocyanin accumulation. By the amino acid structure and phenotypic manifestation (regulation of anthocyanin accumulation in leaves), Myb114 B. oleracea is the closest to Ant2 tomato, Myb2 eggplant and Myb113-like2 pepper alleles. A high degree of conservatism of the SANT DNA-binding domains of MYB114 TF of B. oleracea and R2R3-MYB TF of nightshades was established.

Keywords

Brassica oleracea varieties, anthocyanin biosynthesis, R2R3-MYB transcription factors, TF domains, sequencing, DNA markers.

References

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