The Membrane Nanodomain Flot1 Protein Participates in Formation of the Early Endosomes in the Root Cells of Arabidopsis thaliana
- Autores: Khalilova L.1, Voronkov A.1
-
Afiliações:
- Timiryazev Institute of Plant Physiology, Russian Academy of Sciences
- Edição: Volume 70, Nº 4 (2023)
- Páginas: 382-391
- Seção: ЭКСПЕРИМЕНТАЛЬНЫЕ СТАТЬИ
- URL: https://journals.rcsi.science/0015-3303/article/view/139780
- DOI: https://doi.org/10.31857/S0015330323600067
- EDN: https://elibrary.ru/QAHCTE
- ID: 139780
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Resumo
Plants are subjected to various stress factors within their lifespan. In this respect, the plasma membrane
is a principal cell compartment responsible for plant adaptations to stresses. It is capable of remodeling
its protein composition by means of endocytosis. In the plants, the main mode of this process is a clathrinmediated
endocytosis. Several clathrin-independent pathways are also known; these alternative mechanisms
involve Flot1 protein. In the present research, the role of Flot1 in the endocytosis process was examined in
seedling roots of a wild type and an Atflot1ko knockout mutant of Arabidopsis thaliana (L.) Heynh. Light
microscopy with an FM4-64 lipophilic probe and transmission electron microscopy were used. It was found
that endocytosis was arrested in the root cells of the wild type after a simultaneous treatment of the roots with
an inhibitor of clathrin-mediated endocytosis (1-naphthylacetic acid) and the agent depleting the plasma
membrane of sterols (methyl-β-cyclodextrin). In this case, such morphological change as reduction in cytoplasm
vesiculation (including the early endosomes, the small vesicles originated from the agranular ER, the
microvacuoles from its fragments, and the clathrin vesicles) was observed. The vesiculation was diminished
in both the control and the stressed plants (exposed to 100 mM NaCl). In the Atflot1ko mutant, the cisterns
of the Golgi complex closed up to a ring, and the process of formation of the early endosomes was completely
abolished under these conditions. It is suggested that, in the roots of A. thaliana exposed to the inhibitors, the
microdomain-associated Flot1 protein of the plasma membrane conserves the structure of the Golgi complex
and its capacity to build early endosomes on the trans-side. In addition, the protein appears to participate in
formation of the early endosomes from the trans-Golgi network.
Sobre autores
L. Khalilova
Timiryazev Institute of Plant Physiology, Russian Academy of Sciences
Email: lhalilova@mail.ru
Moscow
A. Voronkov
Timiryazev Institute of Plant Physiology, Russian Academy of Sciences
Autor responsável pela correspondência
Email: lhalilova@mail.ru
Moscow
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