A Method for Purification and Modification of a Bone Xenotransplant Material in Biphase Media Containing High-Pressure CO2

Abstract

A promising method was proposed for the first time for purification of animal bone tissue fragments from high-molecular weight compounds in biphase media containing high-pressure CO₂ (solutions of carbonic and peroxycarbonic acids). The efficiency of this purification was confirmed by SEM and IR spectroscopy. It turned out that, during the exposure of bone fragments in solutions of carbonic and peroxycarbonic acids at high CO₂ pressure, mineral phase components of bone tissue are also extracted, which can be used to obtain highly dispersed hydroxyapatite particles upon extract reprecipitation. The structure and composition of the extracts were investigated by X-ray diffraction and scanning electron microscopy (SEM).