电邮这篇文章 Detection of Low-Abundant MicroRNAs with Hybridization Microchips
- 作者: Shkurnikov M.1, Knyazev E.2, Fomicheva K.1, Kasatkin M.3, Arkov V.4, Makarova Y.1
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隶属关系:
- P. A. Hertsen Moscow Oncology Research Center, Branch of National, Medical Research Radiological Center, Ministry of Health of the Russian Federation
- BioClinicum Company
- Department of Sports Medicine and Medical Rehabilitation, I. M. Sechenov First Moscow State Medical University (Sechenov University), Ministry of Health of the Russian Federation
- Branch No. 1 of the Moscow Scientific-Practical, Center for Medical Rehabilitation, Reconstructive and Sports Medicine
- 期: 卷 166, 编号 6 (2019)
- 页面: 788-792
- 栏目: Experimental Biology
- URL: https://journals.rcsi.science/0007-4888/article/view/241370
- DOI: https://doi.org/10.1007/s10517-019-04441-z
- ID: 241370
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详细
The effect of low concentrations of miRNA on the ability of GeneChip miRNA 4.0 hybridization chips to evaluate their representation in the sample was studied. It is shown that the evaluation of the expression of 61 miRNAs is statistically significantly associated with the multiplicity of plasma dilution. Only 12 miRNAs showed very high Pearson correlation coefficient (>0.95) and they all decreased in response to dilution. High abundance of has-miR-4532 miRNA in plasma was demonstrated. This miRNA was never detected during sequencing of similar samples. It was concluded that in case of miRNA expression <1.12±0.33 units in log2 scale, dilution was not followed by further decrease in the signal intensity in GeneChip miRNA 4.0 chips.
作者简介
M. Shkurnikov
P. A. Hertsen Moscow Oncology Research Center, Branch of National, Medical Research Radiological Center, Ministry of Health of the Russian Federation
编辑信件的主要联系方式.
Email: mshkurnikov@imscs.msu.ru
俄罗斯联邦, Moscow
E. Knyazev
BioClinicum Company
Email: mshkurnikov@imscs.msu.ru
俄罗斯联邦, Moscow
K. Fomicheva
P. A. Hertsen Moscow Oncology Research Center, Branch of National, Medical Research Radiological Center, Ministry of Health of the Russian Federation
Email: mshkurnikov@imscs.msu.ru
俄罗斯联邦, Moscow
M. Kasatkin
Department of Sports Medicine and Medical Rehabilitation, I. M. Sechenov First Moscow State Medical University (Sechenov University), Ministry of Health of the Russian Federation
Email: mshkurnikov@imscs.msu.ru
俄罗斯联邦, Moscow
V. Arkov
Branch No. 1 of the Moscow Scientific-Practical, Center for Medical Rehabilitation, Reconstructive and Sports Medicine
Email: mshkurnikov@imscs.msu.ru
俄罗斯联邦, Moscow
Yu. Makarova
P. A. Hertsen Moscow Oncology Research Center, Branch of National, Medical Research Radiological Center, Ministry of Health of the Russian Federation
Email: mshkurnikov@imscs.msu.ru
俄罗斯联邦, Moscow
