Email this article Detection of Low-Abundant MicroRNAs with Hybridization Microchips
- Authors: Shkurnikov M.Y.1, Knyazev E.N.2, Fomicheva K.A.1, Kasatkin M.S.3, Arkov V.V.4, Makarova Y.A.1
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Affiliations:
- P. A. Hertsen Moscow Oncology Research Center, Branch of National, Medical Research Radiological Center, Ministry of Health of the Russian Federation
- BioClinicum Company
- Department of Sports Medicine and Medical Rehabilitation, I. M. Sechenov First Moscow State Medical University (Sechenov University), Ministry of Health of the Russian Federation
- Branch No. 1 of the Moscow Scientific-Practical, Center for Medical Rehabilitation, Reconstructive and Sports Medicine
- Issue: Vol 166, No 6 (2019)
- Pages: 788-792
- Section: Experimental Biology
- URL: https://journals.rcsi.science/0007-4888/article/view/241370
- DOI: https://doi.org/10.1007/s10517-019-04441-z
- ID: 241370
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Abstract
The effect of low concentrations of miRNA on the ability of GeneChip miRNA 4.0 hybridization chips to evaluate their representation in the sample was studied. It is shown that the evaluation of the expression of 61 miRNAs is statistically significantly associated with the multiplicity of plasma dilution. Only 12 miRNAs showed very high Pearson correlation coefficient (>0.95) and they all decreased in response to dilution. High abundance of has-miR-4532 miRNA in plasma was demonstrated. This miRNA was never detected during sequencing of similar samples. It was concluded that in case of miRNA expression <1.12±0.33 units in log2 scale, dilution was not followed by further decrease in the signal intensity in GeneChip miRNA 4.0 chips.
About the authors
M. Yu. Shkurnikov
P. A. Hertsen Moscow Oncology Research Center, Branch of National, Medical Research Radiological Center, Ministry of Health of the Russian Federation
Author for correspondence.
Email: mshkurnikov@imscs.msu.ru
Russian Federation, Moscow
E. N. Knyazev
BioClinicum Company
Email: mshkurnikov@imscs.msu.ru
Russian Federation, Moscow
K. A. Fomicheva
P. A. Hertsen Moscow Oncology Research Center, Branch of National, Medical Research Radiological Center, Ministry of Health of the Russian Federation
Email: mshkurnikov@imscs.msu.ru
Russian Federation, Moscow
M. S. Kasatkin
Department of Sports Medicine and Medical Rehabilitation, I. M. Sechenov First Moscow State Medical University (Sechenov University), Ministry of Health of the Russian Federation
Email: mshkurnikov@imscs.msu.ru
Russian Federation, Moscow
V. V. Arkov
Branch No. 1 of the Moscow Scientific-Practical, Center for Medical Rehabilitation, Reconstructive and Sports Medicine
Email: mshkurnikov@imscs.msu.ru
Russian Federation, Moscow
Yu. A. Makarova
P. A. Hertsen Moscow Oncology Research Center, Branch of National, Medical Research Radiological Center, Ministry of Health of the Russian Federation
Email: mshkurnikov@imscs.msu.ru
Russian Federation, Moscow
