Мақаланы E-mail арқылы жіберу Detection of Low-Abundant MicroRNAs with Hybridization Microchips
- Авторлар: Shkurnikov M.1, Knyazev E.2, Fomicheva K.1, Kasatkin M.3, Arkov V.4, Makarova Y.1
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Мекемелер:
- P. A. Hertsen Moscow Oncology Research Center, Branch of National, Medical Research Radiological Center, Ministry of Health of the Russian Federation
- BioClinicum Company
- Department of Sports Medicine and Medical Rehabilitation, I. M. Sechenov First Moscow State Medical University (Sechenov University), Ministry of Health of the Russian Federation
- Branch No. 1 of the Moscow Scientific-Practical, Center for Medical Rehabilitation, Reconstructive and Sports Medicine
- Шығарылым: Том 166, № 6 (2019)
- Беттер: 788-792
- Бөлім: Experimental Biology
- URL: https://journals.rcsi.science/0007-4888/article/view/241370
- DOI: https://doi.org/10.1007/s10517-019-04441-z
- ID: 241370
Дәйексөз келтіру
Ашық рұқсат
Рұқсат берілді
Тек жазылушылар үшін
Аннотация
The effect of low concentrations of miRNA on the ability of GeneChip miRNA 4.0 hybridization chips to evaluate their representation in the sample was studied. It is shown that the evaluation of the expression of 61 miRNAs is statistically significantly associated with the multiplicity of plasma dilution. Only 12 miRNAs showed very high Pearson correlation coefficient (>0.95) and they all decreased in response to dilution. High abundance of has-miR-4532 miRNA in plasma was demonstrated. This miRNA was never detected during sequencing of similar samples. It was concluded that in case of miRNA expression <1.12±0.33 units in log2 scale, dilution was not followed by further decrease in the signal intensity in GeneChip miRNA 4.0 chips.
Авторлар туралы
M. Shkurnikov
P. A. Hertsen Moscow Oncology Research Center, Branch of National, Medical Research Radiological Center, Ministry of Health of the Russian Federation
Хат алмасуға жауапты Автор.
Email: mshkurnikov@imscs.msu.ru
Ресей, Moscow
E. Knyazev
BioClinicum Company
Email: mshkurnikov@imscs.msu.ru
Ресей, Moscow
K. Fomicheva
P. A. Hertsen Moscow Oncology Research Center, Branch of National, Medical Research Radiological Center, Ministry of Health of the Russian Federation
Email: mshkurnikov@imscs.msu.ru
Ресей, Moscow
M. Kasatkin
Department of Sports Medicine and Medical Rehabilitation, I. M. Sechenov First Moscow State Medical University (Sechenov University), Ministry of Health of the Russian Federation
Email: mshkurnikov@imscs.msu.ru
Ресей, Moscow
V. Arkov
Branch No. 1 of the Moscow Scientific-Practical, Center for Medical Rehabilitation, Reconstructive and Sports Medicine
Email: mshkurnikov@imscs.msu.ru
Ресей, Moscow
Yu. Makarova
P. A. Hertsen Moscow Oncology Research Center, Branch of National, Medical Research Radiological Center, Ministry of Health of the Russian Federation
Email: mshkurnikov@imscs.msu.ru
Ресей, Moscow
