Comparative Study of HIF-1α- and HIF-2α-Immunopositive Neurons and Capillaries in Rat Cortex under Conditions of Tissue Hypoxia
- 作者: Chertok V.1, Nevzorova V.2, Zakharchuk N.2
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隶属关系:
- Department of Human Anatomy, Pacific State Medical University, Ministry of Health of the Russian Federation
- Institute of Hospital Therapy and Diagnostic Tools, Pacific State Medical University, Ministry of Health of the Russian Federation
- 期: 卷 165, 编号 4 (2018)
- 页面: 516-520
- 栏目: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/240479
- DOI: https://doi.org/10.1007/s10517-018-4207-6
- ID: 240479
如何引用文章
详细
We measured the content of HIF-1α and HIF-2α-immunopositive neurons and microvessels in the brain of Wistar rats during the first 24 h of tissue hypoxia induced by subcutaneous injection of cobalt dichloride (50 mg/kg). In control rats (without hypoxia), immunohistochemical marker HIF-2α in cortex of parietal lobe was not detected, and HIF-1α was detected only in few weakly stained pale neurons and capillaries. In 30 min after injection of the cobalt salt, the number of HIF-1α+ neurons increased by 25.6% (in capillaries by 12.3%), many of these were characterized by intensive reaction; the quantitative parameters reached their maximum level within 1-3 h. However, the concentration of immunopositive neurons returned to the control values in 6 h after hypoxia modeling (capillaries in 9 h). In contrast to HIF-1α, the number of neurons and capillaries containing HIF-2α reached a maximum level in 6-12 h of hypoxia. The relative density of HIF-2α+ capillaries increased most pronouncedly (by 23.6%); the relative density of neurons increased by 18.9%. The relative density of HIF-2α+ cells did not change significantly to the end of the experiment. Thus, HIF-1α is more essential for regulation of adaptation to hypoxia in neurons and HIF-2α is more important for the endothelium of microvessels.
作者简介
V. Chertok
Department of Human Anatomy, Pacific State Medical University, Ministry of Health of the Russian Federation
编辑信件的主要联系方式.
Email: chertokv@mail.ru
俄罗斯联邦, Vladivostok
V. Nevzorova
Institute of Hospital Therapy and Diagnostic Tools, Pacific State Medical University, Ministry of Health of the Russian Federation
Email: chertokv@mail.ru
俄罗斯联邦, Vladivostok
N. Zakharchuk
Institute of Hospital Therapy and Diagnostic Tools, Pacific State Medical University, Ministry of Health of the Russian Federation
Email: chertokv@mail.ru
俄罗斯联邦, Vladivostok
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