Comparative Study of HIF-1α- and HIF-2α-Immunopositive Neurons and Capillaries in Rat Cortex under Conditions of Tissue Hypoxia
- Авторлар: Chertok V.1, Nevzorova V.2, Zakharchuk N.2
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Мекемелер:
- Department of Human Anatomy, Pacific State Medical University, Ministry of Health of the Russian Federation
- Institute of Hospital Therapy and Diagnostic Tools, Pacific State Medical University, Ministry of Health of the Russian Federation
- Шығарылым: Том 165, № 4 (2018)
- Беттер: 516-520
- Бөлім: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/240479
- DOI: https://doi.org/10.1007/s10517-018-4207-6
- ID: 240479
Дәйексөз келтіру
Аннотация
We measured the content of HIF-1α and HIF-2α-immunopositive neurons and microvessels in the brain of Wistar rats during the first 24 h of tissue hypoxia induced by subcutaneous injection of cobalt dichloride (50 mg/kg). In control rats (without hypoxia), immunohistochemical marker HIF-2α in cortex of parietal lobe was not detected, and HIF-1α was detected only in few weakly stained pale neurons and capillaries. In 30 min after injection of the cobalt salt, the number of HIF-1α+ neurons increased by 25.6% (in capillaries by 12.3%), many of these were characterized by intensive reaction; the quantitative parameters reached their maximum level within 1-3 h. However, the concentration of immunopositive neurons returned to the control values in 6 h after hypoxia modeling (capillaries in 9 h). In contrast to HIF-1α, the number of neurons and capillaries containing HIF-2α reached a maximum level in 6-12 h of hypoxia. The relative density of HIF-2α+ capillaries increased most pronouncedly (by 23.6%); the relative density of neurons increased by 18.9%. The relative density of HIF-2α+ cells did not change significantly to the end of the experiment. Thus, HIF-1α is more essential for regulation of adaptation to hypoxia in neurons and HIF-2α is more important for the endothelium of microvessels.
Авторлар туралы
V. Chertok
Department of Human Anatomy, Pacific State Medical University, Ministry of Health of the Russian Federation
Хат алмасуға жауапты Автор.
Email: chertokv@mail.ru
Ресей, Vladivostok
V. Nevzorova
Institute of Hospital Therapy and Diagnostic Tools, Pacific State Medical University, Ministry of Health of the Russian Federation
Email: chertokv@mail.ru
Ресей, Vladivostok
N. Zakharchuk
Institute of Hospital Therapy and Diagnostic Tools, Pacific State Medical University, Ministry of Health of the Russian Federation
Email: chertokv@mail.ru
Ресей, Vladivostok
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