Comparative Analysis of the Expression of Glutathione Peroxidase and Glutathione Reductase Genes in Human Sperm after Cryopreservation
- 作者: Makarova N.1, Romanov Y.1, Dolgushina N.1, Parker M.1, Krasnyi A.1
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隶属关系:
- V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
- 期: 卷 165, 编号 1 (2018)
- 页面: 166-170
- 栏目: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/240118
- DOI: https://doi.org/10.1007/s10517-018-4121-y
- ID: 240118
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详细
We analyzed the effect of cryopreservation on the expression of glutathione peroxidase (GPX1) and glutathione reductase (GSR) genes in human sperm cells (15 sperm samples from fertile donors and 10 samples from infertile patients). The relative expression of GPX1 and GSR genes was determined by real-time PCR. The rate of post-thaw recovery was 2.1 times higher in the group of fertile donors. A significant increase in the expression of GPX1, but not GSR, was observed in sperm samples from infertile patients, while in patients with infertility, GPX1 expression significantly decreased after cryopreservation/thawing, in samples from fertile donors after the same procedure it increased to the level observed in the sperm samples from infertile patients. A positive correlation was revealed between GPX1 expression and sperm cryotolerance.
作者简介
N. Makarova
V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: romanov1553@yandex.ru
俄罗斯联邦, Moscow
Yu. Romanov
V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
编辑信件的主要联系方式.
Email: romanov1553@yandex.ru
俄罗斯联邦, Moscow
N. Dolgushina
V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: romanov1553@yandex.ru
俄罗斯联邦, Moscow
M. Parker
V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: romanov1553@yandex.ru
俄罗斯联邦, Moscow
A. Krasnyi
V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: romanov1553@yandex.ru
俄罗斯联邦, Moscow