Comparative Analysis of the Expression of Glutathione Peroxidase and Glutathione Reductase Genes in Human Sperm after Cryopreservation
- Авторлар: Makarova N.1, Romanov Y.1, Dolgushina N.1, Parker M.1, Krasnyi A.1
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Мекемелер:
- V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
- Шығарылым: Том 165, № 1 (2018)
- Беттер: 166-170
- Бөлім: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/240118
- DOI: https://doi.org/10.1007/s10517-018-4121-y
- ID: 240118
Дәйексөз келтіру
Аннотация
We analyzed the effect of cryopreservation on the expression of glutathione peroxidase (GPX1) and glutathione reductase (GSR) genes in human sperm cells (15 sperm samples from fertile donors and 10 samples from infertile patients). The relative expression of GPX1 and GSR genes was determined by real-time PCR. The rate of post-thaw recovery was 2.1 times higher in the group of fertile donors. A significant increase in the expression of GPX1, but not GSR, was observed in sperm samples from infertile patients, while in patients with infertility, GPX1 expression significantly decreased after cryopreservation/thawing, in samples from fertile donors after the same procedure it increased to the level observed in the sperm samples from infertile patients. A positive correlation was revealed between GPX1 expression and sperm cryotolerance.
Авторлар туралы
N. Makarova
V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: romanov1553@yandex.ru
Ресей, Moscow
Yu. Romanov
V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Хат алмасуға жауапты Автор.
Email: romanov1553@yandex.ru
Ресей, Moscow
N. Dolgushina
V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: romanov1553@yandex.ru
Ресей, Moscow
M. Parker
V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: romanov1553@yandex.ru
Ресей, Moscow
A. Krasnyi
V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: romanov1553@yandex.ru
Ресей, Moscow