Effect of Oxidized Dextran on Cytokine Production and Activation of IRF3 Transcription Factor in Macrophages from Mice of Opposite Strains with Different Sensitivity to Tuberculosis Infection
- 作者: Chechushkov A.1, Kozhin P.1, Zaitseva N.1, Gainutdinov P.1, Men’shchikova E.1, Troitskii A.1, Shkurupy V.1
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隶属关系:
- Research Institute of Experimental and Clinical Medicine
- 期: 卷 164, 编号 6 (2018)
- 页面: 738-742
- 栏目: Microbiology and Immunology
- URL: https://journals.rcsi.science/0007-4888/article/view/239888
- DOI: https://doi.org/10.1007/s10517-018-4070-5
- ID: 239888
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详细
We studied differences in the production of pro- and anti-inflammatory cytokines and IRF3 transcription factor by peritoneal macrophages from mice of opposite strains CBA/J and C57Bl/6 and the effect of 60-kDa oxidized dextran on these parameters. Macrophages from C57Bl/6 mice were mainly characterized by the production of proinflammatory cytokines TNFα, IL-12, and MCP-1 (markers of M1 polarization). By contrast, CBA/J mice exhibited a relatively high level of anti-inflammatory cytokine IL-10 and lower expression of proinflammatory cytokines (M2 phenotype). IRF3 content in peritoneal macrophages of CBA/J mice was higher than in C57Bl/6 mice. Oxidized dextran decreased the expression of IRF3 upon stimulation of cells from CBA/J mice with LPS, but increased this process in C57Bl/6 mice. Despite a diversity of oxidized dextran-induced changes in cytokine production, the data confirm our hypothesis that this agent can stimulate the alternative activation of macrophages.
作者简介
A. Chechushkov
Research Institute of Experimental and Clinical Medicine
Email: lemen@centercem.ru
俄罗斯联邦, Novosibirsk
P. Kozhin
Research Institute of Experimental and Clinical Medicine
Email: lemen@centercem.ru
俄罗斯联邦, Novosibirsk
N. Zaitseva
Research Institute of Experimental and Clinical Medicine
Email: lemen@centercem.ru
俄罗斯联邦, Novosibirsk
P. Gainutdinov
Research Institute of Experimental and Clinical Medicine
Email: lemen@centercem.ru
俄罗斯联邦, Novosibirsk
E. Men’shchikova
Research Institute of Experimental and Clinical Medicine
编辑信件的主要联系方式.
Email: lemen@centercem.ru
俄罗斯联邦, Novosibirsk
A. Troitskii
Research Institute of Experimental and Clinical Medicine
Email: lemen@centercem.ru
俄罗斯联邦, Novosibirsk
V. Shkurupy
Research Institute of Experimental and Clinical Medicine
Email: lemen@centercem.ru
俄罗斯联邦, Novosibirsk