Effect of Oxidized Dextran on Cytokine Production and Activation of IRF3 Transcription Factor in Macrophages from Mice of Opposite Strains with Different Sensitivity to Tuberculosis Infection
- Authors: Chechushkov A.V.1, Kozhin P.M.1, Zaitseva N.S.1, Gainutdinov P.I.1, Men’shchikova E.B.1, Troitskii A.V.1, Shkurupy V.A.1
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Affiliations:
- Research Institute of Experimental and Clinical Medicine
- Issue: Vol 164, No 6 (2018)
- Pages: 738-742
- Section: Microbiology and Immunology
- URL: https://journals.rcsi.science/0007-4888/article/view/239888
- DOI: https://doi.org/10.1007/s10517-018-4070-5
- ID: 239888
Cite item
Abstract
We studied differences in the production of pro- and anti-inflammatory cytokines and IRF3 transcription factor by peritoneal macrophages from mice of opposite strains CBA/J and C57Bl/6 and the effect of 60-kDa oxidized dextran on these parameters. Macrophages from C57Bl/6 mice were mainly characterized by the production of proinflammatory cytokines TNFα, IL-12, and MCP-1 (markers of M1 polarization). By contrast, CBA/J mice exhibited a relatively high level of anti-inflammatory cytokine IL-10 and lower expression of proinflammatory cytokines (M2 phenotype). IRF3 content in peritoneal macrophages of CBA/J mice was higher than in C57Bl/6 mice. Oxidized dextran decreased the expression of IRF3 upon stimulation of cells from CBA/J mice with LPS, but increased this process in C57Bl/6 mice. Despite a diversity of oxidized dextran-induced changes in cytokine production, the data confirm our hypothesis that this agent can stimulate the alternative activation of macrophages.
About the authors
A. V. Chechushkov
Research Institute of Experimental and Clinical Medicine
Email: lemen@centercem.ru
Russian Federation, Novosibirsk
P. M. Kozhin
Research Institute of Experimental and Clinical Medicine
Email: lemen@centercem.ru
Russian Federation, Novosibirsk
N. S. Zaitseva
Research Institute of Experimental and Clinical Medicine
Email: lemen@centercem.ru
Russian Federation, Novosibirsk
P. I. Gainutdinov
Research Institute of Experimental and Clinical Medicine
Email: lemen@centercem.ru
Russian Federation, Novosibirsk
E. B. Men’shchikova
Research Institute of Experimental and Clinical Medicine
Author for correspondence.
Email: lemen@centercem.ru
Russian Federation, Novosibirsk
A. V. Troitskii
Research Institute of Experimental and Clinical Medicine
Email: lemen@centercem.ru
Russian Federation, Novosibirsk
V. A. Shkurupy
Research Institute of Experimental and Clinical Medicine
Email: lemen@centercem.ru
Russian Federation, Novosibirsk