Peculiarities of Cell Seeding on Polylactic Acid-Based Scaffolds Fabricated Using Electrospinning and Solution Blow Spinning Technologies
- 作者: Afanasiev S.1, Muslimova E.1, Nashchekina Y.2, Nikonov P.2, Rogovskaya Y.1, Bolbasov E.3, Tverdokhlebov S.3
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隶属关系:
- Research Institute of Cardiology, Tomsk National Research Medical Center, Russian Academy of Sciences
- Institute of Cytology, Russian Academy of Sciences
- National Research Tomsk Polytechnical University
- 期: 卷 164, 编号 2 (2017)
- 页面: 281-284
- 栏目: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://journals.rcsi.science/0007-4888/article/view/239553
- DOI: https://doi.org/10.1007/s10517-017-3973-x
- ID: 239553
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详细
We studied the possibility of seeding bone marrow-derived stromal cells onto polylactic acid-based scaffolds fabricated by electrospinning and solution blow spinning technologies. The cells were applied to the scaffolds by dynamic seeding and scaffolds were then cultured in Petri dishes in culture medium for 3 days. Cell migration to the Petri dish surface was noted only for scaffolds fabricated by electrospinning technology, but DAPI staining confirmed the presence of cells in both scaffolds. The mean number of cells in scaffolds fabricated by electrospinning and solution blow spinning was 56±9 and 81±6, respectively. The scaffold fabricated by solution blow spinning was more effectively (p<0.05) colonized by cells due to its more optimal spatial structure.
作者简介
S. Afanasiev
Research Institute of Cardiology, Tomsk National Research Medical Center, Russian Academy of Sciences
编辑信件的主要联系方式.
Email: tursky@cardio-tomsk.ru
俄罗斯联邦, Tomsk
E. Muslimova
Research Institute of Cardiology, Tomsk National Research Medical Center, Russian Academy of Sciences
Email: tursky@cardio-tomsk.ru
俄罗斯联邦, Tomsk
Yu. Nashchekina
Institute of Cytology, Russian Academy of Sciences
Email: tursky@cardio-tomsk.ru
俄罗斯联邦, St. Petersburg
P. Nikonov
Institute of Cytology, Russian Academy of Sciences
Email: tursky@cardio-tomsk.ru
俄罗斯联邦, St. Petersburg
Yu. Rogovskaya
Research Institute of Cardiology, Tomsk National Research Medical Center, Russian Academy of Sciences
Email: tursky@cardio-tomsk.ru
俄罗斯联邦, Tomsk
E. Bolbasov
National Research Tomsk Polytechnical University
Email: tursky@cardio-tomsk.ru
俄罗斯联邦, Tomsk
S. Tverdokhlebov
National Research Tomsk Polytechnical University
Email: tursky@cardio-tomsk.ru
俄罗斯联邦, Tomsk