Inhibition of the Expression of Inducible NO Synthase by Neuroactive Amino Acid Derivatives Phenibut and Glufimet In Vitro and Ex Vivo
- 作者: Borisov A.1, Prokofiev I.1, Mokrousov I.1, Perfilova V.1, Tyurenkov I.1
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隶属关系:
- Department of Pharmacology and Biopharmacia, Faculty of Continuing Medical Education, Volgograd State Medical University, Ministry of Health of the Russian Federation
- 期: 卷 164, 编号 2 (2017)
- 页面: 177-180
- 栏目: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/239458
- DOI: https://doi.org/10.1007/s10517-017-3952-2
- ID: 239458
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详细
The effects of glufimet and phenibut (glutamic acid and GABA derivatives, respectively) on concentration of inducible NO synthase and cGMP in LPS-activated mouse peritoneal macrophages and on NO end products in their culture medium were examined in vitro and ex vivo. Addition of LPS into culture medium elevated concentration of NO metabolites in this medium and increased concentration of inducible NO synthase and cGMP in the lysates of peritoneal macrophages, whereas incubation of the cells with examined agents applied at concentration of 10—5 M diminished these indices. Similar results were obtained with intraperitoneal injection of LPS, glufimet, and phenibut. In culture medium containing peritoneal macrophages from the mice injected with LPS (100 μg/kg), the concentrations of inducible NO synthase and cGMP as well as the total concentration of nitrite and nitrate ions increased, whereas in culture medium with the cells from LPS-exposed mice treated with glufimet (28.7 mg/kg) and phenibut (50 mg/kg) these indices significantly decreased.
作者简介
A. Borisov
Department of Pharmacology and Biopharmacia, Faculty of Continuing Medical Education, Volgograd State Medical University, Ministry of Health of the Russian Federation
Email: igor.prokofiev@mail.ru
俄罗斯联邦, Moscow
I. Prokofiev
Department of Pharmacology and Biopharmacia, Faculty of Continuing Medical Education, Volgograd State Medical University, Ministry of Health of the Russian Federation
编辑信件的主要联系方式.
Email: igor.prokofiev@mail.ru
俄罗斯联邦, Moscow
I. Mokrousov
Department of Pharmacology and Biopharmacia, Faculty of Continuing Medical Education, Volgograd State Medical University, Ministry of Health of the Russian Federation
Email: igor.prokofiev@mail.ru
俄罗斯联邦, Moscow
V. Perfilova
Department of Pharmacology and Biopharmacia, Faculty of Continuing Medical Education, Volgograd State Medical University, Ministry of Health of the Russian Federation
Email: igor.prokofiev@mail.ru
俄罗斯联邦, Moscow
I. Tyurenkov
Department of Pharmacology and Biopharmacia, Faculty of Continuing Medical Education, Volgograd State Medical University, Ministry of Health of the Russian Federation
Email: igor.prokofiev@mail.ru
俄罗斯联邦, Moscow