Oligonucleotide Microchip for the Identification of Infectious Agents of Reproductive System with Simultaneous Analysis of Determinants of Resistance to Antimicrobial Substances
- 作者: Leinsoo A.1, Shaskol’skii B.1, Dement’eva E.1, Gryadunov D.1, Kubanov A.2, Chestkov A.2, Obraztsova O.2, Shpilevaya M.2, Deryabin D.2
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隶属关系:
- V. A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
- State Research Center of Dermatovenerology and Cosmetology, Ministry of Health of the Russian Federation
- 期: 卷 164, 编号 1 (2017)
- 页面: 54-60
- 栏目: Immunology and Microbiology
- URL: https://journals.rcsi.science/0007-4888/article/view/239357
- DOI: https://doi.org/10.1007/s10517-017-3925-5
- ID: 239357
如何引用文章
详细
We developed a multiplexed DNA microarray-based assay allowing identification of 12 causative agents of reproductive tract infections with the simultaneous detection of 47 genetic determinants of resistance to antimicrobial substances. The microarray was tested on 93 isolates of Neisseria gonorrhoeae, 32 isolates of Treponema pallidum and 29 samples of Ureaplasma spp./Mycoplasma spp. The N. gonorrhoeae isolates had multiple mutations in the penA, ponA, rpsJ, gyrA, parC, and mtrR genes; their prognostic value significantly increased when combinations of mutations were detected. In the analyzed T. pallidum isolates, single A2058G substitution in the 23S rRNA gene responsible for macrolide resistance was found. DNA sequences of Ureaplasma spp./Mycoplasma spp. were determined as wild type, which was not fully consistent with the results of analysis of their antimicrobial susceptibility
作者简介
A. Leinsoo
V. A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: b.shaskolskiy@biochip.ru
俄罗斯联邦, Moscow
B. Shaskol’skii
V. A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
编辑信件的主要联系方式.
Email: b.shaskolskiy@biochip.ru
俄罗斯联邦, Moscow
E. Dement’eva
V. A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: b.shaskolskiy@biochip.ru
俄罗斯联邦, Moscow
D. Gryadunov
V. A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: b.shaskolskiy@biochip.ru
俄罗斯联邦, Moscow
A. Kubanov
State Research Center of Dermatovenerology and Cosmetology, Ministry of Health of the Russian Federation
Email: b.shaskolskiy@biochip.ru
俄罗斯联邦, Moscow
A. Chestkov
State Research Center of Dermatovenerology and Cosmetology, Ministry of Health of the Russian Federation
Email: b.shaskolskiy@biochip.ru
俄罗斯联邦, Moscow
O. Obraztsova
State Research Center of Dermatovenerology and Cosmetology, Ministry of Health of the Russian Federation
Email: b.shaskolskiy@biochip.ru
俄罗斯联邦, Moscow
M. Shpilevaya
State Research Center of Dermatovenerology and Cosmetology, Ministry of Health of the Russian Federation
Email: b.shaskolskiy@biochip.ru
俄罗斯联邦, Moscow
D. Deryabin
State Research Center of Dermatovenerology and Cosmetology, Ministry of Health of the Russian Federation
Email: b.shaskolskiy@biochip.ru
俄罗斯联邦, Moscow
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