Oligonucleotide Microchip for the Identification of Infectious Agents of Reproductive System with Simultaneous Analysis of Determinants of Resistance to Antimicrobial Substances
- Authors: Leinsoo A.T.1, Shaskol’skii B.L.1, Dement’eva E.I.1, Gryadunov D.A.1, Kubanov A.A.2, Chestkov A.V.2, Obraztsova O.A.2, Shpilevaya M.V.2, Deryabin D.G.2
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Affiliations:
- V. A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
- State Research Center of Dermatovenerology and Cosmetology, Ministry of Health of the Russian Federation
- Issue: Vol 164, No 1 (2017)
- Pages: 54-60
- Section: Immunology and Microbiology
- URL: https://journals.rcsi.science/0007-4888/article/view/239357
- DOI: https://doi.org/10.1007/s10517-017-3925-5
- ID: 239357
Cite item
Abstract
We developed a multiplexed DNA microarray-based assay allowing identification of 12 causative agents of reproductive tract infections with the simultaneous detection of 47 genetic determinants of resistance to antimicrobial substances. The microarray was tested on 93 isolates of Neisseria gonorrhoeae, 32 isolates of Treponema pallidum and 29 samples of Ureaplasma spp./Mycoplasma spp. The N. gonorrhoeae isolates had multiple mutations in the penA, ponA, rpsJ, gyrA, parC, and mtrR genes; their prognostic value significantly increased when combinations of mutations were detected. In the analyzed T. pallidum isolates, single A2058G substitution in the 23S rRNA gene responsible for macrolide resistance was found. DNA sequences of Ureaplasma spp./Mycoplasma spp. were determined as wild type, which was not fully consistent with the results of analysis of their antimicrobial susceptibility
About the authors
A. T. Leinsoo
V. A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: b.shaskolskiy@biochip.ru
Russian Federation, Moscow
B. L. Shaskol’skii
V. A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Author for correspondence.
Email: b.shaskolskiy@biochip.ru
Russian Federation, Moscow
E. I. Dement’eva
V. A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: b.shaskolskiy@biochip.ru
Russian Federation, Moscow
D. A. Gryadunov
V. A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: b.shaskolskiy@biochip.ru
Russian Federation, Moscow
A. A. Kubanov
State Research Center of Dermatovenerology and Cosmetology, Ministry of Health of the Russian Federation
Email: b.shaskolskiy@biochip.ru
Russian Federation, Moscow
A. V. Chestkov
State Research Center of Dermatovenerology and Cosmetology, Ministry of Health of the Russian Federation
Email: b.shaskolskiy@biochip.ru
Russian Federation, Moscow
O. A. Obraztsova
State Research Center of Dermatovenerology and Cosmetology, Ministry of Health of the Russian Federation
Email: b.shaskolskiy@biochip.ru
Russian Federation, Moscow
M. V. Shpilevaya
State Research Center of Dermatovenerology and Cosmetology, Ministry of Health of the Russian Federation
Email: b.shaskolskiy@biochip.ru
Russian Federation, Moscow
D. G. Deryabin
State Research Center of Dermatovenerology and Cosmetology, Ministry of Health of the Russian Federation
Email: b.shaskolskiy@biochip.ru
Russian Federation, Moscow