Lentiviral Transduction of Neurons in Adult Brain: Evaluation of Inflammatory Response and Cognitive Effects in Mice
- 作者: Kunitsyna T.1, Ivashkina O.1, Roshchina M.1, Toropova K.1, Anokhin K.1
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隶属关系:
- National Research Center Kurchatov Institute
- 期: 卷 161, 编号 2 (2016)
- 页面: 316-319
- 栏目: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/237382
- DOI: https://doi.org/10.1007/s10517-016-3404-4
- ID: 237382
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详细
We evaluated the effect of hippocampal injection of lentiviral particles p156-CMV-EGFP on behavior, learning, and microglial Iba1+ cells activation in mice. Testing in the open field and elevated plus-maze revealed higher anxiety levels in lentiviral-injected mice in comparison with animals injected with vehicle. At the same time, lentivirus injection did not change learning and memory of mice in the hippocampal-dependent fear conditioning task. Microglia density in lentivirus-injected mice was significantly higher than in vehicle-injected mice. Thus, hippocampal injection of lentiviral particles with minimum content of transgenes produced evident inflammation process, changed anxiety level of experimental animals, but had no effect on hippocampal-dependent learning and memory.
作者简介
T. Kunitsyna
National Research Center Kurchatov Institute
Email: oivashkina@gmail.com
俄罗斯联邦, Moscow
O. Ivashkina
National Research Center Kurchatov Institute
编辑信件的主要联系方式.
Email: oivashkina@gmail.com
俄罗斯联邦, Moscow
M. Roshchina
National Research Center Kurchatov Institute
Email: oivashkina@gmail.com
俄罗斯联邦, Moscow
K. Toropova
National Research Center Kurchatov Institute
Email: oivashkina@gmail.com
俄罗斯联邦, Moscow
K. Anokhin
National Research Center Kurchatov Institute
Email: oivashkina@gmail.com
俄罗斯联邦, Moscow