A Model of Cadmium Uptake and Transport in Caco-2 Cells
- 作者: Gerasimenko T.1, Senyavina N.1, Anisimov N.2, Tonevitskaya S.2
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隶属关系:
- BioCilicum Research and Production Center
- Moscow State University of Mechanical Engineering (MAMI)
- 期: 卷 161, 编号 1 (2016)
- 页面: 187-192
- 栏目: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://journals.rcsi.science/0007-4888/article/view/237258
- DOI: https://doi.org/10.1007/s10517-016-3373-7
- ID: 237258
如何引用文章
详细
We created a physiologically substantiated kinetic model of cadmium transport and toxicity in intestinal cell model (Caco-2 cells). Transcriptome profiling of Caco-2 cells revealed high content of transporter DMT1 and ZIP14 and intensive expression of some calcium channels of the CACN family. The mathematical model describing three types of transporters, as well as intracellular cadmium binding with metallothionein and excretion through the basolateral membrane allowed us to construct cadmium uptake and transport curves that approximated the previously obtained experimental data. Using the proposed model, we determined toxic intracellular cadmium concentration leading to cell death and impairing the integrity of cell monolayer and described cadmium transport in this case.
作者简介
T. Gerasimenko
BioCilicum Research and Production Center
Email: n.seniavina@bioclinicum.com
俄罗斯联邦, Moscow
N. Senyavina
BioCilicum Research and Production Center
编辑信件的主要联系方式.
Email: n.seniavina@bioclinicum.com
俄罗斯联邦, Moscow
N. Anisimov
Moscow State University of Mechanical Engineering (MAMI)
Email: n.seniavina@bioclinicum.com
俄罗斯联邦, Moscow
S. Tonevitskaya
Moscow State University of Mechanical Engineering (MAMI)
Email: n.seniavina@bioclinicum.com
俄罗斯联邦, Moscow