Mathematical and Experimental Model of Oxygen Diffusion for HepaRG Cell Spheroids
- 作者: Aleksandrova A.1, Pulkova N.2, Gerasimenko T.1, Anisimov N.2, Tonevitskaya S.2, Sakharov D.1
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隶属关系:
- BioClinicum Research and Development Center
- Moscow State University of Mechanical Engineering (MAMI)
- 期: 卷 160, 编号 6 (2016)
- 页面: 857-860
- 栏目: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/237015
- DOI: https://doi.org/10.1007/s10517-016-3326-1
- ID: 237015
如何引用文章
详细
3D cell cultures are extensively used to study in vitro toxic effect of xenobiotics. When using multicellular spheroids, the question about their optimal size should be solved: small spheroids are difficult to manipulate, while large size of spheroids impairs the transport of nutrients and oxygen into the center. Mathematical models describing the distribution of substances in multicellular spheroids numerical procedure for solving differential equation system, which complicates their use in laboratory practice. We proposed and experimentally evaluated a new mathematical model describing oxygen distribution in HepaRG cell spheroids. Markers of functional activity were studied in spheroids of different size. The maximum size of spheroids that can be maintained in culture for 9 days without necrosis was determined.
作者简介
A. Aleksandrova
BioClinicum Research and Development Center
编辑信件的主要联系方式.
Email: a.aleksandrova@bioclinicum.com
俄罗斯联邦, Moscow
N. Pulkova
Moscow State University of Mechanical Engineering (MAMI)
Email: a.aleksandrova@bioclinicum.com
俄罗斯联邦, Moscow
T. Gerasimenko
BioClinicum Research and Development Center
Email: a.aleksandrova@bioclinicum.com
俄罗斯联邦, Moscow
N. Anisimov
Moscow State University of Mechanical Engineering (MAMI)
Email: a.aleksandrova@bioclinicum.com
俄罗斯联邦, Moscow
S. Tonevitskaya
Moscow State University of Mechanical Engineering (MAMI)
Email: a.aleksandrova@bioclinicum.com
俄罗斯联邦, Moscow
D. Sakharov
BioClinicum Research and Development Center
Email: a.aleksandrova@bioclinicum.com
俄罗斯联邦, Moscow
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