Mathematical and Experimental Model of Oxygen Diffusion for HepaRG Cell Spheroids
- Authors: Aleksandrova A.V.1, Pulkova N.P.2, Gerasimenko T.N.1, Anisimov N.Y.2, Tonevitskaya S.A.2, Sakharov D.A.1
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Affiliations:
- BioClinicum Research and Development Center
- Moscow State University of Mechanical Engineering (MAMI)
- Issue: Vol 160, No 6 (2016)
- Pages: 857-860
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/237015
- DOI: https://doi.org/10.1007/s10517-016-3326-1
- ID: 237015
Cite item
Abstract
3D cell cultures are extensively used to study in vitro toxic effect of xenobiotics. When using multicellular spheroids, the question about their optimal size should be solved: small spheroids are difficult to manipulate, while large size of spheroids impairs the transport of nutrients and oxygen into the center. Mathematical models describing the distribution of substances in multicellular spheroids numerical procedure for solving differential equation system, which complicates their use in laboratory practice. We proposed and experimentally evaluated a new mathematical model describing oxygen distribution in HepaRG cell spheroids. Markers of functional activity were studied in spheroids of different size. The maximum size of spheroids that can be maintained in culture for 9 days without necrosis was determined.
About the authors
A. V. Aleksandrova
BioClinicum Research and Development Center
Author for correspondence.
Email: a.aleksandrova@bioclinicum.com
Russian Federation, Moscow
N. P. Pulkova
Moscow State University of Mechanical Engineering (MAMI)
Email: a.aleksandrova@bioclinicum.com
Russian Federation, Moscow
T. N. Gerasimenko
BioClinicum Research and Development Center
Email: a.aleksandrova@bioclinicum.com
Russian Federation, Moscow
N. Yu. Anisimov
Moscow State University of Mechanical Engineering (MAMI)
Email: a.aleksandrova@bioclinicum.com
Russian Federation, Moscow
S. A. Tonevitskaya
Moscow State University of Mechanical Engineering (MAMI)
Email: a.aleksandrova@bioclinicum.com
Russian Federation, Moscow
D. A. Sakharov
BioClinicum Research and Development Center
Email: a.aleksandrova@bioclinicum.com
Russian Federation, Moscow
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