Detection of Antileukocytic Antibodies in Blood Serum using Lymphocytes and Latex Microspheres Carrying HLA-Antigens upon Alloimmunization of Women with Recurrent Pregnancy Loss
- 作者: Stepanova E.1, Nikolaeva M.1, Golubeva E.1, Vtorushina V.1, Van’ko L.1, Khodzhaeva Z.1, Krechetova L.1
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隶属关系:
- V. I. Kulakov Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
- 期: 卷 160, 编号 5 (2016)
- 页面: 722-726
- 栏目: Methods
- URL: https://journals.rcsi.science/0007-4888/article/view/236918
- DOI: https://doi.org/10.1007/s10517-016-3260-2
- ID: 236918
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详细
Anti-HLA-antibodies were detected using cross-reaction of blood serum with allogenic T and B cells and latex microspheres coated with HLA-I and HLA-II antigens. HLA+ and HLA–sera obtained from women before and after allogeneic immunization were tested. The results obtained by these methods significantly differed. The test with latex microspheres detected antibodies to HLA-I and HLA-II antigens with high sensitivity and specificity and can be used for assessment of clinical significance of alloantibody detection when using alloimmunization in the therapy of gestation disorders.
作者简介
E. Stepanova
V. I. Kulakov Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
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Email: elena2404.07@mail.ru
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M. Nikolaeva
V. I. Kulakov Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: elena2404.07@mail.ru
俄罗斯联邦, Moscow
E. Golubeva
V. I. Kulakov Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: elena2404.07@mail.ru
俄罗斯联邦, Moscow
V. Vtorushina
V. I. Kulakov Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: elena2404.07@mail.ru
俄罗斯联邦, Moscow
L. Van’ko
V. I. Kulakov Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: elena2404.07@mail.ru
俄罗斯联邦, Moscow
Z. Khodzhaeva
V. I. Kulakov Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: elena2404.07@mail.ru
俄罗斯联邦, Moscow
L. Krechetova
V. I. Kulakov Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: elena2404.07@mail.ru
俄罗斯联邦, Moscow