Detection of Antileukocytic Antibodies in Blood Serum using Lymphocytes and Latex Microspheres Carrying HLA-Antigens upon Alloimmunization of Women with Recurrent Pregnancy Loss
- Autores: Stepanova E.1, Nikolaeva M.1, Golubeva E.1, Vtorushina V.1, Van’ko L.1, Khodzhaeva Z.1, Krechetova L.1
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Afiliações:
- V. I. Kulakov Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
- Edição: Volume 160, Nº 5 (2016)
- Páginas: 722-726
- Seção: Methods
- URL: https://journals.rcsi.science/0007-4888/article/view/236918
- DOI: https://doi.org/10.1007/s10517-016-3260-2
- ID: 236918
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Resumo
Anti-HLA-antibodies were detected using cross-reaction of blood serum with allogenic T and B cells and latex microspheres coated with HLA-I and HLA-II antigens. HLA+ and HLA–sera obtained from women before and after allogeneic immunization were tested. The results obtained by these methods significantly differed. The test with latex microspheres detected antibodies to HLA-I and HLA-II antigens with high sensitivity and specificity and can be used for assessment of clinical significance of alloantibody detection when using alloimmunization in the therapy of gestation disorders.
Sobre autores
E. Stepanova
V. I. Kulakov Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Autor responsável pela correspondência
Email: elena2404.07@mail.ru
Rússia, Moscow
M. Nikolaeva
V. I. Kulakov Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: elena2404.07@mail.ru
Rússia, Moscow
E. Golubeva
V. I. Kulakov Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: elena2404.07@mail.ru
Rússia, Moscow
V. Vtorushina
V. I. Kulakov Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: elena2404.07@mail.ru
Rússia, Moscow
L. Van’ko
V. I. Kulakov Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: elena2404.07@mail.ru
Rússia, Moscow
Z. Khodzhaeva
V. I. Kulakov Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: elena2404.07@mail.ru
Rússia, Moscow
L. Krechetova
V. I. Kulakov Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: elena2404.07@mail.ru
Rússia, Moscow