Results of Experimental Ligation of the Main Vein with the Use of Cell Technologies
- Autores: Maiborodin I.1, Morozov V.1, Matveeva V.1, Anikeev A.1, Figurenko N.1, Maslov R.1, Chastikin G.1, Maiborodina V.2
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Afiliações:
- Center of New Medical Technologies, Institute of Chemical Biology and Basic Medicine, Siberian Division of the Russian Academy of Sciences
- Laboratory of Ultrastructural Bases of Pathology, Institute of Molecular Pathology and Pathomorphology
- Edição: Volume 164, Nº 1 (2017)
- Páginas: 61-67
- Seção: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/239361
- DOI: https://doi.org/10.1007/s10517-017-3926-4
- ID: 239361
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Resumo
Autologous multipotent mesenchymal stromal cells (MMSC) of bone marrow origin with transfected GFP gene and additionally stained cell membranes were injected to rats through the skin in the projection of ligated femoral vein. The results were evaluated by fluorescent microscopy. No signs of MMSC incorporation into the wall of ligated vessel or reorganized collaterals were detected. Angiogenesis processes involving MMSC were detected in experimental rats within just 4 days and progressed until week 2 postinjection, mainly in granulations at the site of surgical intervention and the cicatrix forming there. Injected MMSC completely formed all tunics of the new vessels and incorporated in the vessels forming from the recipient cells. MMSC and the objects created from them were gradually eliminated with participation of macrophages and replaced by structures formed from the recipient cells.
Sobre autores
I. Maiborodin
Center of New Medical Technologies, Institute of Chemical Biology and Basic Medicine, Siberian Division of the Russian Academy of Sciences
Autor responsável pela correspondência
Email: imai@mail.ru
Rússia, Novosibirsk
V. Morozov
Center of New Medical Technologies, Institute of Chemical Biology and Basic Medicine, Siberian Division of the Russian Academy of Sciences
Email: imai@mail.ru
Rússia, Novosibirsk
V. Matveeva
Center of New Medical Technologies, Institute of Chemical Biology and Basic Medicine, Siberian Division of the Russian Academy of Sciences
Email: imai@mail.ru
Rússia, Novosibirsk
A. Anikeev
Center of New Medical Technologies, Institute of Chemical Biology and Basic Medicine, Siberian Division of the Russian Academy of Sciences
Email: imai@mail.ru
Rússia, Novosibirsk
N. Figurenko
Center of New Medical Technologies, Institute of Chemical Biology and Basic Medicine, Siberian Division of the Russian Academy of Sciences
Email: imai@mail.ru
Rússia, Novosibirsk
R. Maslov
Center of New Medical Technologies, Institute of Chemical Biology and Basic Medicine, Siberian Division of the Russian Academy of Sciences
Email: imai@mail.ru
Rússia, Novosibirsk
G. Chastikin
Center of New Medical Technologies, Institute of Chemical Biology and Basic Medicine, Siberian Division of the Russian Academy of Sciences
Email: imai@mail.ru
Rússia, Novosibirsk
V. Maiborodina
Laboratory of Ultrastructural Bases of Pathology, Institute of Molecular Pathology and Pathomorphology
Email: imai@mail.ru
Rússia, Novosibirsk