Results of Experimental Ligation of the Main Vein with the Use of Cell Technologies
- Authors: Maiborodin I.V.1, Morozov V.V.1, Matveeva V.A.1, Anikeev A.A.1, Figurenko N.F.1, Maslov R.V.1, Chastikin G.A.1, Maiborodina V.I.2
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Affiliations:
- Center of New Medical Technologies, Institute of Chemical Biology and Basic Medicine, Siberian Division of the Russian Academy of Sciences
- Laboratory of Ultrastructural Bases of Pathology, Institute of Molecular Pathology and Pathomorphology
- Issue: Vol 164, No 1 (2017)
- Pages: 61-67
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/239361
- DOI: https://doi.org/10.1007/s10517-017-3926-4
- ID: 239361
Cite item
Abstract
Autologous multipotent mesenchymal stromal cells (MMSC) of bone marrow origin with transfected GFP gene and additionally stained cell membranes were injected to rats through the skin in the projection of ligated femoral vein. The results were evaluated by fluorescent microscopy. No signs of MMSC incorporation into the wall of ligated vessel or reorganized collaterals were detected. Angiogenesis processes involving MMSC were detected in experimental rats within just 4 days and progressed until week 2 postinjection, mainly in granulations at the site of surgical intervention and the cicatrix forming there. Injected MMSC completely formed all tunics of the new vessels and incorporated in the vessels forming from the recipient cells. MMSC and the objects created from them were gradually eliminated with participation of macrophages and replaced by structures formed from the recipient cells.
About the authors
I. V. Maiborodin
Center of New Medical Technologies, Institute of Chemical Biology and Basic Medicine, Siberian Division of the Russian Academy of Sciences
Author for correspondence.
Email: imai@mail.ru
Russian Federation, Novosibirsk
V. V. Morozov
Center of New Medical Technologies, Institute of Chemical Biology and Basic Medicine, Siberian Division of the Russian Academy of Sciences
Email: imai@mail.ru
Russian Federation, Novosibirsk
V. A. Matveeva
Center of New Medical Technologies, Institute of Chemical Biology and Basic Medicine, Siberian Division of the Russian Academy of Sciences
Email: imai@mail.ru
Russian Federation, Novosibirsk
A. A. Anikeev
Center of New Medical Technologies, Institute of Chemical Biology and Basic Medicine, Siberian Division of the Russian Academy of Sciences
Email: imai@mail.ru
Russian Federation, Novosibirsk
N. F. Figurenko
Center of New Medical Technologies, Institute of Chemical Biology and Basic Medicine, Siberian Division of the Russian Academy of Sciences
Email: imai@mail.ru
Russian Federation, Novosibirsk
R. V. Maslov
Center of New Medical Technologies, Institute of Chemical Biology and Basic Medicine, Siberian Division of the Russian Academy of Sciences
Email: imai@mail.ru
Russian Federation, Novosibirsk
G. A. Chastikin
Center of New Medical Technologies, Institute of Chemical Biology and Basic Medicine, Siberian Division of the Russian Academy of Sciences
Email: imai@mail.ru
Russian Federation, Novosibirsk
V. I. Maiborodina
Laboratory of Ultrastructural Bases of Pathology, Institute of Molecular Pathology and Pathomorphology
Email: imai@mail.ru
Russian Federation, Novosibirsk